Oxytocin (OT) is essential for parturition and milk ejection, and OT-containing fibers are present in several regions of the brain and in the spinal cord. During lactation, activation of spinal cord neurons by suckling stimulation involves deep laminae III-X including sympathetic preganglionic neurons of the intermedio-medial cell column. In the present study, experiments were designed to determine if the suckling provided by the litter increased OT levels in the spinal cord of dams, as determined by competitive immunoassay. In addition, we investigated if OT fibers reach neurons of the spinal cord that are known to respond to suckling. The OT content was higher in the hypothalamus than in the spinal cord in animals from all experimental groups. After 6 h of pup separation, OT levels decreased and suckling for 5 min induced a significant increase of OT levels in the spinal cord. Double immunostaining for Fos and OT showed OT-positive fibers adjacent to neurons that had Fos-positive nuclei, located mostly in laminae III, IV, and X. The present data support the notion that OT is released within the spinal cord in response to suckling, suggesting a role for this peptide in modulating the afferent and/or efferent responses generated by suckling.

译文

催产素 (OT) 对于分娩和乳汁排出至关重要,并且含OT的纤维存在于大脑和脊髓的多个区域。在哺乳期,哺乳刺激激活脊髓神经元涉及深层iii-x,包括中间细胞柱的交感神经节前神经元。在本研究中,设计了实验来确定垫料提供的哺乳是否增加了大坝脊髓中的OT水平,这是通过竞争性免疫测定法确定的。此外,我们研究了OT纤维是否到达已知对哺乳有反应的脊髓神经元。在所有实验组的动物中,下丘脑的OT含量均高于脊髓。幼崽分离6小时后,OT水平降低,哺乳5分钟后脊髓中OT水平显着增加。Fos和OT的双重免疫染色显示OT阳性纤维与具有Fos阳性核的神经元相邻,主要位于laminae III,IV和X中。目前的数据支持OT响应哺乳而在脊髓内释放的观点,这表明该肽在调节哺乳产生的传入和/或传出反应中的作用。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录