Intercellular adhesion in some parenchyma becomes strengthened in response to stress. The present study provides an approach to investigate this phenomenon (usually attributed to pectin methyl esterase and binding of Ca(2+) and/or rhamnogalacturonan-II-borate) through reliable stress simulation by probing organic acid molecules in potato tuber parenchyma. Short-chain monocarboxylic acids induce consistent intercellular adhesion strengthening (3.8-5.3 newton) at pH >or= 3 < pK(a), where pectin methyl esterase activity and Ca(2+) or borate binding are limited, and vice versa at pH > pK(a) with a strength of 1.4-2.0 newton as compared to 0.3-0.4 newton for the nonincubated control. Strengthening of intercellular adhesion is characterized by prominent staining of pectin and protein and immunogold labeling of pectin in the cell wall and the middle lamellar complex, particularly after boiling. Pectin confers strengthening to the primary cell wall, as reflected by: (i) prominent immunogold labeling following boiling; and (ii) puncturing macerated cells by starch gelatinization pressure after enzymatic pectin removal.

译文

一些薄壁组织的细胞间粘附在应激反应中得到加强。本研究提供了一种方法,通过探测马铃薯块茎薄壁组织中的有机酸分子,通过可靠的应力模拟来研究这种现象 (通常归因于果胶甲基酯酶和Ca(2) 和/或鼠李糖半乳糖醛酸-II-硼酸酯的结合)。短链单羧酸在pH> 或 = 3 < pK(a) 时诱导一致的细胞间粘附增强 (3.8-5.3牛顿),其中果胶甲基酯酶活性和Ca(2) 或硼酸盐结合受到限制,与未孵育对照的0.3-0.4牛顿相比,pH> pK(a) 的强度为1.4-2.0牛顿,反之亦然。增强细胞间粘附的特征是果胶和蛋白质的显着染色以及果胶在细胞壁和中层状复合物中的免疫金标记,尤其是在煮沸后。果胶赋予增强原代细胞壁的力量,这反映在 :( i) 沸腾后显着的免疫金标记; (ii) 在酶促果胶去除后,通过淀粉糊化压力刺穿浸软细胞。

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