Staphylococcus aureus (S. aureus) causes persistent biofilm-related infections. Biofilm formation by S. aureus is affected by the culture conditions and is associated with certain genotypic characteristics. Here, we show that glucose and sodium chloride (NaCl) supplementation of culture media, a common practice in studies of biofilms in vitro, influences both biofilm formation by 40 S. aureus clinical isolates (methicillin-resistant and methicillin-sensitive S. aureus) and causes variations in biofilm quantification. Methicillin-resistant strains formed more robust biofilms than methicillin-sensitive strains in tryptic soy broth (TSB). However, glucose supplementation in TSB greatly promoted and stabilized biofilm formation of all strains, while additional NaCl was less efficient in this respect and resulted in significant variation in biofilm measurements. In addition, we observed that the ST239-SCCmec (Staphylococcal Cassette Chromosome mec) type III lineage formed strong biofilms in TSB supplemented with glucose and NaCl. Links between biofilm formation and accessory gene regulator (agr) status, as assessed by δ-toxin production, and with mannitol fermentation were not found. Our results show that TSB supplemented with 1.0% glucose supports robust biofilm production and reproducible quantification of S. aureus biofilm formation in vitro, whereas additional NaCl results in major variations in measurements of biofilm formation.

译文

金黄色葡萄球菌 (S. aureus) 引起持续的生物膜相关感染。金黄色葡萄球菌的生物膜形成受培养条件的影响,并与某些基因型特征有关。在这里,我们显示了培养基的葡萄糖和氯化钠 (NaCl) 补充,这是体外生物膜研究的常见做法,影响了40株金黄色葡萄球菌临床分离株 (耐甲氧西林和甲氧西林敏感金黄色葡萄球菌) 的生物膜形成,并导致生物膜定量的变化。在胰蛋白酶大豆肉汤 (TSB) 中,耐甲氧西林的菌株比对甲氧西林敏感的菌株形成更坚固的生物膜。然而,TSB中的葡萄糖补充极大地促进和稳定了所有菌株的生物膜形成,而额外的NaCl在这方面的效率较低,并导致生物膜测量的显着变化。此外,我们观察到ST239-SCCmec (葡萄球菌盒染色体mec) III型谱系在补充了葡萄糖和NaCl的TSB中形成了强生物膜。未发现通过 δ 毒素产生评估的生物膜形成与辅助基因调节剂 (agr) 状态以及甘露醇发酵之间的联系。我们的结果表明,补充有1.0% 葡萄糖的TSB支持体外稳定的生物膜产生和金黄色葡萄球菌生物膜形成的可重复定量,而额外的NaCl导致生物膜形成的测量结果的主要变化。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录