Spheroids are three-dimensional cell aggregates expressing histotypic organisation in vitro comparable to tissue continuity in vivo. They can be prepared from normal tissue and from tumour fragments. In the experiments presented here, dermal human spheroids and brain tumour spheroids are prepared from the same patient. The dermal tissue originates from the border of the incision wound made to effect a stereotactic brain tumour biopsy. The tumour originates from a fragment of the collected stereotactic biopsy. The dermal fragment and the brain biopsy are explanted in vitro to form confluent monolayers. At confluency, the dermal cells are transferred into small Erlenmeyer flasks and rotated at 37 degrees C for 1-2 days and rotation mediated spheroids are formed. Small flaps of the tumour monolayer are placed on a semisolid non-adhesive substrate, reorganise and form agar overlay spheroids. After spheroid formation, a dermal spheroid is confronted with a brain tumour derived spheroid. The confronting pair, after adhering to each other, present an invasion model in vitro. The dermal spheroid functions as the autologous host for the brain tumour spheroid. Putative invasive cells present in the reaggregated brain spheroid will invade the dermal spheroid and destroy it. If no invasive cells are present in the tumour derived spheroid no morphologic changes will be seen in the dermal spheroid; 24 tested brain biopsy spheroids demonstrated a clear correlation between malignancy in situ and invasiveness in vitro. So it can be concluded that the autologous confrontation of brain tumour derived spheroids with dermal spheroids derived from the patient has a predictive value concerning malignant evolution and mimics the situation of the tumour in situ.

译文

球体是三维细胞聚集体,在体外表达组织类型,与体内组织连续性相当。它们可以从正常组织和肿瘤碎片中制备。在这里介绍的实验中,皮肤人球体和脑肿瘤球体是由同一患者制备的。真皮组织起源于切口伤口的边界,以进行立体定向脑肿瘤活检。肿瘤起源于收集的立体定向活检的片段。在体外移植真皮碎片和脑活检以形成汇合的单层。汇合时,将真皮细胞转移到小锥形瓶中,并在37 ℃ 旋转1-2天,并形成旋转介导的球体。将肿瘤单层的小皮瓣放置在半固体非粘附基质上,重组并形成琼脂覆盖球体。球体形成后,真皮球体面对脑肿瘤衍生的球体。相互粘附后,面对的一对在体外呈现入侵模型。真皮球体充当脑肿瘤球体的自体宿主。在重新聚集的大脑球体中存在的假定的侵入性细胞将侵入真皮球体并破坏它。如果肿瘤衍生的球体中没有浸润性细胞,则真皮球体中不会出现形态学变化; 24个经过测试的脑活检球体表明原位恶性肿瘤与体外侵袭性之间存在明显的相关性。因此可以得出结论,脑肿瘤衍生的球体与患者衍生的真皮球体的自体对抗具有有关恶性演变的预测价值,并模仿了原位肿瘤的情况。

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