Culture-based studies of the microbial community within the gut of the medicinal leech have typically been focused on various Aeromonas species, which were believed to be the sole symbiont of the leech digestive tract. In this study, analysis of 16S rRNA gene clone libraries confirmed the presence of Aeromonas veronii and revealed a second symbiont, clone PW3, a novel member of the Rikenellaceae, within the crop, a large compartment where ingested blood is stored prior to digestion. The diversity of the bacterial community in the leech intestinum was determined, and additional symbionts were detected, including members of the alpha-, gamma-, and delta-Proteobacteria, Fusobacteria, Firmicutes, and Bacteroidetes. The relative abundances of the clones suggested that A. veronii and the novel clone, PW3, also dominate the intestinum community, while other clones, representing transient organisms, were typically present in low numbers. The identities of these transients varied greatly between individual leeches. Neither time after feeding nor feeding on defibrinated blood caused a change in identity of the dominant members of the microbial communities. Terminal restriction fragment length polymorphism analysis was used to verify that the results from the clone libraries were representative of a larger data set. The presence of a two-member bacterial community in the crop provides a unique opportunity to investigate both symbiont-symbiont and symbiont-host interactions in a natural model of digestive-tract associations.

译文

对药用水蛭肠道内微生物群落的基于培养的研究通常集中在各种气单胞菌物种上,这些物种被认为是水蛭消化道的唯一共生体。在这项研究中,对16S rRNA基因克隆文库的分析证实了veronii气单胞菌的存在,并揭示了第二个共生体,即Rikenellaceae的新成员clone PW3,在作物内有一个大的隔间,在消化之前会储存摄入的血液。确定了水蛭肠道中细菌群落的多样性,并检测到了其他共生体,包括 α-,γ-和 δ-变形菌,梭菌,厚壁菌和拟杆菌的成员。克隆的相对丰度表明,A. veronii和新型克隆PW3也主导着肠道群落,而代表瞬态生物的其他克隆通常以少量存在。这些瞬变的身份在各个水ches之间差异很大。喂食或喂食脱纤维的血液后的时间都不会导致微生物群落主要成员的身份发生变化。末端限制性片段长度多态性分析用于验证克隆库的结果代表较大的数据集。作物中两人细菌群落的存在为在消化道关联的自然模型中研究共生体-共生体和共生体-宿主相互作用提供了独特的机会。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录