This study investigated in vitro endocrine disrupting effects of three mixtures of POPs: 'Marine mix' extracted from Atlantic cod liver, and two mixtures extracted from burbot liver, 'Mjøsa mix' and 'Losna mix'. The POP mixtures were chemically characterized. Co-culture of theca and granulosa cells, were exposed for 48h with different doses of 'Marine mix', 'Mjøsa mix' or 'Losna mix'. As an end point cell viability was determinated by LDH test, steroid analysis by EIA and caspase-3 by colorimetric substrate. Chemical characterization of the mixtures demonstrated that the 'Marine mix' contained high levels of DDTs and PCBs. In the 'Mjøsa mix', the dominant pollutants were BDEs and HBCD. The concentrations of POPs measured in the 'Losna mix' were considerably lower. All mixtures used in the present study had a stimulatory effect on testosterone and estradiol secretion with 'Marine mix'>'Mjøsa mix'>'Losna mix'. These results show that even a mixture containing background concentrations of POPs significantly affected steroid secretion. A higher steroidogenic response in low dose ranges, compared with high dose ranges indicated xenobiotic-conditioning hormesis. This could complicate predictions of effects in risk assessments.

译文

这项研究调查了三种持久性有机污染物混合物的体外内分泌干扰作用: 从大西洋鳕鱼肝中提取的 “海洋混合物”,以及从burbot肝脏中提取的两种混合物 “mj ø samix” 和 “losna mix”。POP混合物经过化学表征。用不同剂量的 “海洋混合物”,“mj ø samix” 或 “losna混合物” 暴露卵泡膜和颗粒细胞的共培养48小时。作为终点,通过LDH测试,通过EIA进行类固醇分析并通过比色底物caspase-3测定细胞活力。混合物的化学特征表明,“海洋混合物” 中含有高水平的滴滴涕和多氯联苯。在 “mj ø samix” 中,主要污染物是溴二苯醚和六溴环十二烷。在 “losna混合物” 中测得的POPs浓度要低得多。本研究中使用的所有混合物均通过 “海洋混合物”> “mj ø samix”> “losna混合物” 对睾丸激素和雌二醇的分泌具有刺激作用。这些结果表明,即使是含有背景浓度的持久性有机污染物的混合物也会显着影响类固醇的分泌。与高剂量范围相比,低剂量范围内较高的类固醇生成反应表明异源生物调节功能。这可能会使风险评估中影响的预测复杂化。

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