Acute kidney injury is followed by regeneration of damaged renal tubular epithelial cells. The purpose of this study was to test the hypothesis that renal stem cells exist in the adult kidney and participate in the repair process. A unique population of cells that behave in a manner that is consistent with a renal stem cell were isolated from rat kidneys and were termed multipotent renal progenitor cells (MRPC). Features of these cells include spindle-shaped morphology; self-renewal for >200 population doublings without evidence for senescence; normal karyotype and DNA analysis; and expression of vimentin, CD90 (thy1.1), Pax-2, and Oct4 but not cytokeratin, MHC class I or II, or other markers of more differentiated cells. MRPC exhibit plasticity that is demonstrated by the ability of the cells to be induced to express endothelial, hepatocyte, and neural markers by reverse transcriptase-PCR and immunohistochemistry. The cells can differentiate into renal tubules when injected under the capsule of an uninjured kidney or intra-arterially after renal ischemia-reperfusion injury. Oct4 expression was seen in some tubular cells in the adult kidney, suggesting these cells may be candidate renal stem cells. It is proposed that MRPC participate in the regenerative response of the kidney to acute injury.

译文

急性肾损伤后,受损的肾小管上皮细胞再生。目的检验肾干细胞存在于成人肾脏并参与修复过程的假说。从大鼠肾脏中分离出一种独特的细胞群,其行为方式与肾干细胞一致,被称为多能肾祖细胞 (MRPC)。这些细胞的特征包括梭形形态;> 200种群倍增的自我更新,没有衰老的证据; 正常的核型和DNA分析; 波形蛋白,CD90 (thy1.1),Pax-2和10月4的表达,而不是细胞角蛋白,MHC I或II类,或更多分化细胞的其他标记。MRPC表现出可塑性,通过逆转录酶PCR和免疫组织化学诱导细胞表达内皮,肝细胞和神经标志物的能力证明了这种可塑性。当在未受伤的肾脏的胶囊下注射或在肾缺血再灌注损伤后动脉内注射时,细胞可以分化为肾小管。在成人肾脏的某些肾小管细胞中观察到10月4的表达,表明这些细胞可能是候选的肾干细胞。建议MRPC参与肾脏对急性损伤的再生反应。

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