Isolated adrenal cells from Vitamin E-deficient and control rats were prepared by a trypsin digestion method. Cyclic adenosine 3',5'-monophosphate (cyclic AMP) formation was studied in response to adrenocorticotropin (ACTH) in the presence and absence of ascorbate by measuring the conversion of prelabeled adenosine 5'-triphosphate [14C]ATP to cyclic [14C]AMP. Ascorbate (0.5 mM) inhibited ACTH-induced cyclic [14C]AMP formation in adrenal cells isolated from Vitamin E-deficient rats but had no effect in the control cells. The inhibitory effect of ascorbate on ACTH-induced cyclic AMP formation in Vitamin E-deficient rats decreased as the concentration of ACTH increased. In Vitamin E-deficient rats ascorbate inhibited ACTH-induced cyclic [14C]AMP formation after 30 min of incubation. There was no further significant accumulation of cyclic [14C]AMP at 60 min or 120 min although in the absence of ascorbate cyclic [14C]AMP continued to be formed. The in vitro addition of alpha-tocopherol reduced the inhibition of ACTH-induced cyclic [14C]AMP formation by ascorbate in Vitamin E-deficient rats. These studies suggest that alpha-tocopherol and ascorbate may affect ACTH-induced cyclic AMP formation through interaction with the membrane-bound enzyme adenylate cyclase.

译文

通过胰蛋白酶消化法从缺乏维生素e的大鼠和对照大鼠中分离出的肾上腺细胞。环腺苷3 ',通过测量预标记的5'-三磷酸腺苷 [14C]ATP向环状 [14C] 的转化,研究了在存在和不存在抗坏血酸的情况下对促肾上腺皮质激素 (ACTH) 的响应5 '-单磷酸 (环状AMP) 的形成。抗坏血酸 (0.5 mM) 抑制ACTH诱导的环状 [14C] 从缺乏维生素e的大鼠中分离出的肾上腺细胞中AMP的形成,但在对照细胞中没有作用。抗坏血酸对缺乏维生素e的大鼠中ACTH诱导的环状AMP形成的抑制作用随着ACTH浓度的增加而降低。在缺乏维生素e的大鼠中,抗坏血酸抑制ACTH诱导的环状 [培养30分钟后形成14C]AMP。在60分钟或120分钟时没有进一步显著的循环 [14C]AMP积累,尽管在没有抗坏血酸环 [14C]AMP继续形成。α-生育酚的体外添加降低了ACTH诱导的循环的抑制 [14C] 抗坏血酸在缺乏维生素e的大鼠中形成AMP。这些研究表明,α-生育酚和抗坏血酸可能通过与膜结合酶腺苷酸环化酶的相互作用影响ACTH诱导的环状AMP形成。

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