The use of a fluorescent dye to visualize cellular material on surfaces offers a targeted sampling approach for locating touch DNA on casework items. However, the current application of such dye is not feasible for examination of relatively large items. As a result, development of an efficient dye application system is required to translate this approach into practice. Here, the spray pattern (area covered, intensity, and evenness) of 15 different commercial spray devices was examined visually using food coloring. From this, five devices were selected to apply Diamond Nucleic Acid Dye (DD) to three substrates (glass slide, plastic sheet, and brown packing tape) seeded with saliva and touch DNA. The cellular material was visualized using the Dino-lite Microscope and Polilight. The inhibitory effects of DD afforded by each spray device were examined using Identifiler Plus® DNA profiling kit and a DNA input of 800 pg. The two most promising devices were further tested on a range of mock casework items seeded with touch DNA. The results presented demonstrate the feasibility of a spray system to apply DD to large surfaces and subsequently detect cellular material at both micro and macroscale. Specifically, the data suggest that a pressurized continuous-spray system is favorable and that droplet size influences the intensity of fluorescence and surface coverage. Furthermore, this study indicates that full STR profiles can be obtained following spraying with DD solution, even with excessive application, which is essential for the widespread use of these devices in casework.

译文

使用荧光染料可视化表面上的细胞材料提供了一种有针对性的采样方法,用于在案例项目上定位触摸DNA。然而,目前这种染料的应用对于检查相对较大的物品是不可行的。因此,需要开发有效的染料应用系统以将这种方法转化为实践。在这里,使用食用色素目视检查了15种不同商业喷雾设备的喷雾模式 (覆盖面积,强度和均匀度)。从中,选择了五种设备将金刚石核酸染料 (DD) 施加到三种接种有唾液和触摸DNA的基材 (载玻片,塑料片和棕色包装带) 上。使用Dino-lite显微镜和Polilight可视化细胞材料。使用Identifiler Plus检查了每个喷雾装置提供的DD的抑制作用。®DNA分析试剂盒和800 pg的DNA输入。这两种最有前途的设备在一系列带有触摸DNA的模拟案例项目上进行了进一步测试。给出的结果证明了喷涂系统将DD应用于大表面并随后在微观和宏观尺度上检测细胞材料的可行性。具体而言,数据表明加压连续喷雾系统是有利的,并且液滴尺寸会影响荧光强度和表面覆盖率。此外,这项研究表明,即使过量使用DD溶液,也可以在喷洒DD溶液后获得完整的STR轮廓,这对于在案例工作中广泛使用这些设备至关重要。

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