We compared the transcriptional activities of the circular and linear forms of short (4kbp) and giant (106kbp) DNA molecules in the presence of a polyamine, spermine (4+). With an increase in the spermine concentration, transcriptional activity was enhanced, followed by an inhibitory effect, and complete inhibition was observed in the sole case of long, linear templates. A difference between the transcriptional properties of circular and linear conformations is found for giant DNA molecules. These results are discussed in relation to DNA conformational transitions.

译文

:我们比较了在存在多胺精胺的情况下,短(4kbp)和巨大(106kbp)DNA分子的环状和线性形式的转录活性。随着精胺浓度的增加,转录活性增强,随后产生抑制作用,在长而线性模板的唯一情况下,观察到完全抑制。发现巨型DNA分子的环状和线性构象的转录特性之间存在差异。讨论了有关DNA构象转变的这些结果。

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