The spermine analogue N1,N4-bis-(2,3-butadienyl)-1,4-butanediamine (MDL-72527), an effective inhibitor of polyamine oxidases (PAOs), triggers a systemic response in tomato (Solanum lycopersicum L.) exposed to sublethal (100mM) and lethal (250mM) NaCl concentrations. The accumulation of free polyamines (PAs), the terminal oxidation of PAs by diamine oxidases (DAOs) and PAOs, and the production of H2O2 by PA oxidases depends on the intensity of salt stress. Spermidine and spermine content increased significantly under sublethal salt concentrations, but remained low under lethal salt stress. Along with increased expression of the selected SlDAO1 and SlPAO1 genes in the leaves and roots, respectively, DAO and PAO activities and their product, H2O2, increased and initiated cell death by irreversible loss of electrolytes at 250mM NaCl. MDL-72527 significantly increased spermine, spermidine and/or putrescine contents as a result of reduced activity of PA oxidases; furthermore, it inhibited H2O2 and NO production during salt treatment. These results indicate that PAO contributed to H2O2 and NO production under salt stress, and the terminal activities of DAO and PAO play a role in cell death induction at 250mM NaCl. However, the inhibition of PAO by MDL-72527 does not increase the salt tolerance of plants, since electrolyte leakage increased significantly in the presence of the inhibitor.

译文

:精胺类似物N1,N4-双-(2,3-丁二烯基)-1,4-丁二胺(MDL-72527)是多胺氧化酶(PAOs)的有效抑制剂,可引发番茄(茄属植物Solanum lycopersicum L. )暴露于亚致死浓度(100mM)和致死浓度(250mM)的NaCl中。游离多胺(PAs)的积累,二胺氧化酶(DAOs)和PAOs对PAs的最终氧化作用以及PA氧化酶产生H2O2取决于盐胁迫的强度。在亚致死盐浓度下,亚精胺和亚精胺含量显着增加,但在致死盐胁迫下,亚精胺和亚精胺含量却保持较低水平。分别在叶片和根中增加所选的SlDAO1和SlPAO1基因的表达,DAO和PAO的活性及其产物H2O2在250mM NaCl下通过不可逆的电解质损失而增加并引发细胞死亡。由于PA氧化酶活性降低,MDL-72527显着增加了精胺,亚精胺和/或腐胺的含量;此外,它还抑制了盐处理过程中的H2O2和NO生成。这些结果表明,PAO促进了盐胁迫下H2O2和NO的产生,而DAO和PAO的末端活性在250mM NaCl诱导细胞死亡中起作用。但是,通过MDL-72527抑制PAO不会增加植物的耐盐性,因为在存在抑制剂的情况下电解质的泄漏会明显增加。

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