Two experimental systems representative of the mitochondrial and death receptor apoptotic pathways are the dexamethasone-induced programmed cell death in mouse thymocytes and the antibody-mediated cross-ligation of the Fas receptor in the human leukemic T-cell line Jurkat, respectively. In both cell systems, caspase-9, -8, and -3 were activated upon induction of apoptosis and a sub-G(1) peak appeared as a sign of ongoing DNA fragmentation. Addition of 1 mM spermine together with dexamethasone inhibited caspase activation and the appearance of the sub-G(1) peak in mouse thymocytes. In contrast, Fas-induced cell death was totally unaffected by spermine addition. Spermine addition significantly elevated the spermine concentration in both thymocytes and Jurkat cells. Thus, spermine per se did not inhibit the caspases but rather their activation. The fact that spermine inhibited caspase activation only in the thymocytes implies that spermine inhibited dexamethasone-induced apoptosis upstream of caspase-9 activation.

译文

:代表线粒体和死亡受体凋亡途径的两个实验系统分别是地塞米松诱导的小鼠胸腺细胞程序性细胞死亡和人白血病T细胞系Jurkat中Fas受体的抗体介导的交叉连接。在这两个细胞系统中,caspase-9,-8和-3在诱导凋亡后均被激活,sub-G(1)峰似乎是正在进行的DNA片段化的迹象。 1 mM精胺与地塞米松一起抑制小鼠胸腺细胞中的caspase活化和sub-G(1)峰的出现。相反,Fas诱导的细胞死亡完全不受精胺添加的影响。添加精胺可显着提高胸腺细胞和Jurkat细胞中的精胺浓度。因此,精胺本身并不抑制胱天蛋白酶,而是抑制它们的活化。精胺仅在胸腺细胞中抑制caspase活化的事实表明,精胺在caspase-9活化的上游抑制了地塞米松诱导的细胞凋亡。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录