Mammalian cells respond to their substrates by complex changes in gene expression profiles, morphology, proliferation and migration. We report that substrate nanotopography alters morpohology and proliferation of human embryonic stem cells (hESCs). Fibronectin-coated poly(di-methyl siloxane) substrates with line-grating (600nm ridges with 600nm spacing and 600+/-150nm feature height) induced hESC alignment and elongation, mediated the organization of cytoskeletal components including actin, vimentin, and alpha-tubulin, and reduced proliferation. Spatial polarization of gamma-tubulin complexes was also observed in response to nanotopography. Furthermore, the addition of actin disrupting agents attenuated the alignment and proliferative effects of nanotopography. These findings further demonstrate the importance of interplay between cytoskeleton and substrate interactions as a key modulator of morphological and proliferative cellular response in hESCs on nanotopography.

译文

:哺乳动物细胞通过基因表达谱,形态,增殖和迁移的复杂变化来响应其底物。我们报告说,基底纳米形貌改变了人类胚胎干细胞(hESCs)的形态和增殖。纤连蛋白涂层的聚二甲基硅氧烷底物具有线栅(间距为600nm,间距为600nm,特征高度为600 / -150nm),诱导hESC对准和伸长,介导了包括肌动蛋白,波形蛋白和α-微管蛋白的细胞骨架成分的组织,并减少扩散。响应纳米形貌还观察到了γ-微管蛋白复合物的空间极化。此外,肌动蛋白破坏剂的添加减弱了纳米形貌的排列和增殖作用。这些发现进一步证明了细胞骨架和底物相互作用之间相互作用的重要性,这是纳米地形图上hESCs中形态学和增殖性细胞应答的关键调节剂。

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