The effect of cis-diamminedichloroplatinum (cisplatin: CDDP) on mouse spermatozoa was evaluated quantitatively by means of equilibrium sedimentation in Percoll. CDDP was administered subcutaneously at doses of 1, 3, and 10 mg/kg/week for 5 weeks. After different periods (1, 3, and 10 weeks) without CDDP, the quality of epididymal sperm was evaluated by sperm count, motility, morphology of sperm, and the apparent density of sperm. At 10 mg/kg dose, about 80% mortality occurred during the administration period. There were no sperm even 10 weeks after discontinuing CDDP. With the 3 mg/kg dose, sperm count, motility, and normal morphology of sperm declined after 1 and 5 weeks, but recovered to the control level after 10 weeks. The sperm distribution profiles in the Percoll gradient were quite different among the groups. The control sperm showed two separated peaks in the gradient, whereas the peak of sperm at 1 and 3 mg/kg were shifted forward to lighter apparent density. CDDP causes a reduction in sperm apparent density and impairs semen quality in mice.

译文

:通过Percoll中的平衡沉淀法定量评估了顺式二甲基二氯铂(顺铂:CDDP)对小鼠精子的作用。以1、3和10 mg / kg /周的剂量皮下注射CDDP,持续5周。在没有CDDP的不同时期(1、3和10周)后,通过精子计数,活力,精子形态和精子表观密度评估附睾精子的质量。以10mg / kg的剂量,在给药期间发生约80%的死亡率。停用CDDP后甚至十周也没有精子。以3 mg / kg的剂量,精子数量,活力和正常形态的精子在1和5周后下降,但在10周后恢复到对照组水平。两组间,Percoll梯度中的精子分布情况差异很大。对照精子在梯度中显示出两个分离的峰,而在1和3 mg / kg时精子的峰向前移动,以降低表观密度。 CDDP导致精子表观密度降低,并损害小鼠精液质量。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录