Prostasomes are membranous vesicles present in ejaculated human semen. They are very rich in cholesterol and can interact with spermatozoa. Their physiological roles are still under study. Prostasomes were mixed with liposomes prepared from various lipids, such as N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium (DOTAP), DOTAP/1,2-dipalmytoyl-sn-glycero-3-phosphorylcholine (DPPC, 4:1 molar ratio) and DOTAP/cholesterol (4:1, molar ratio) at different pH values (5-8). The mixing of the lipid phases (fusion) was determined by the relief of octadecyl rhodamine B chloride (R(18)) self-quenching and the radii of the vesicles, by light scattering measurements. The mixing of lipids and the radii of prostasomes were both influenced by the addition of liposome, although in a different manner. The ability of prostasomes (modified by previous treatment with liposomes) to transfer lipid to spermatozoa was also measured. Pretreatment with DOTAP decreased the phenomenon and addition of DPPC abolished it. On the other hand, pretreatment of prostasomes with DOTAP/cholesterol liposomes did not affect the transfer of lipid between prostasome and spermatozoa. Therefore, the ability of vesicles to fuse (or, at least, to exchange the lipid component) was affected by the enrichment in either natural or artificial lipid. This may open new possibilities for the modulation of spermatozoa capacitation and acrosome reaction.

译文

:prosomesomes是射精的人精液中存在的膜状囊泡。它们富含胆固醇,可以与精子相互作用。它们的生理作用仍在研究中。将前体与由各种脂质(例如N- [1-(2,3-二油酰氧基)丙基] -N,N,N-三甲基铵(DOTAP),DOTAP / 1,2-二巴甲酰基-sn-甘油-在不同的pH值(5-8)下,3-磷酸胆碱(DPPC,摩尔比为4:1)和DOTAP /胆固醇(摩尔比为4:1)。脂质相的混合(融合)是通过减轻十八烷基若丹明B氯化物(R(18))自猝灭和囊泡半径(通过光散射测量)来确定的。脂质的混合和前体半径均受脂质体添加的影响,尽管方式不同。还测量了前体(通过先前用脂质体处理修饰的)将脂质转移至精子的能力。用DOTAP预处理可以减少这种现象,并且添加DPPC可以消除这种现象。另一方面,用DOTAP /胆固醇脂质体对前列腺体进行预处理不会影响脂质在前列腺体与精子之间的转移。因此,天然或人工脂质的富集影响了囊泡融合(或至少交换脂质组分)的能力。这可能为调节精子获能和顶体反应开辟新的可能性。

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