Serum amyloid P component (SAP) belongs to the pentraxin family of proteins, members of which are characterized by radial pentameric structure and calcium-dependent ligand binding. SAP is present in all types of amyloidosis and has been shown to bind to several ligands, but the physiological function of this protein has not been fully elucidated. The present study identified and characterized SAP in human semen and immunolocalized it to the male reproductive tract. SAP was also detected in seminal plasma by immunoblotting and purification by affinity chromatography followed by mass spectrometry. According to electroimmunoassay, the concentration of SAP in semen is approximately 2 mg/L, and flow cytometry revealed SAP attached to the surface of spermatozoa. Moreover, immunohistochemistry showed positive staining of spermatozoa, subsets of epithelial cells, and the stroma of accessory male genital glands and testis. Presence of mRNA supports local production of SAP, as shown with reverse transcription polymerase chain reaction. We identified SAP in a new setting - the human male reproductive system. SAP was detected on ejaculated spermatozoa, in seminal plasma and in tissue sections from the male reproductive tract. Further functional studies are needed to explain the role of SAP in human reproduction.

译文

:血清淀粉样蛋白P组分(SAP)属于pentraxin家族蛋白,其成员以放射状五聚体结构和钙依赖性配体结合为特征。 SAP存在于所有类型的淀粉样变性病中,并已显示与几种配体结合,但尚未完全阐明该蛋白的生理功能。本研究鉴定并表征了人类精液中的SAP,并将其免疫定位于男性生殖道。还可以通过免疫印迹法在精浆中检测到SAP,然后通过亲和色谱法和质谱法进行纯化。根据电免疫测定,精液中SAP的浓度约为2 mg / L,流式细胞仪显示SAP附着在精子表面。此外,免疫组化显示精子,上皮细胞亚群以及雄性生殖器官和睾丸间质的阳性染色。 mRNA的存在支持SAP的局部生产,如逆转录聚合酶链反应所示。我们在新的环境-人类雄性生殖系统中确定了SAP。在射精的精子,精浆和雄性生殖道的组织切片中检测到SAP。需要进一步的功能研究来解释SAP在人类生殖中的作用。

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