Spermatogenesis is a process driven by stem cell, where germ cell cycle is under the control of a specific genotype species. Considering that Jundiá (Rhamdia quelen) is a Neotropical catfish with great economical importance and useful experimental model, little information is available on basic aspects of its reproductive biology, especially on spermatogenesis. As a result, this study aimed to characterize the male germ cells, estimate the duration of spermatogenesis and evaluate the expression of selected stem cell genes in Jundiá testis. Similar to other fish species, our results showed a remarkable decrease of germ cell nuclear volume during Jundiá spermatogenesis, particularly from type A undifferentiated to late type B spermatogonia and from diplotene to late spermatids. Using a S-phase marker, bromodeoxyuridine (BrdU), the combined duration of meiotic and spermiogenic phases in this species was estimated in approximately 7 days. This is considered very short when compared to mammals, where spermatogenesis last from 30 to 74 days. Selected stem cell genes were partially sequenced and characterized in Jundiá testis. Expression analysis showed higher plzf and pou5f3 mRNA levels in the cell fractions enriched by type A undifferentiated spermatogonia. These results were further confirmed by in situ hybridization that showed strong signal of plzf and pou5f3 mRNA in type A undifferentiated spermatogonia. Altogether, these information will expand our knowledge of the reproductive biology of this species, contributing to improve its production and management, and also for biotechnological applications, such as germ cell transplantation.

译文

:精子发生是由干细胞驱动的过程,其中生殖细胞的周期受特定基因型物种的控制。考虑到Jundiá(Rhamdia quelen)是一种新热带cat鱼,具有重要的经济意义和有用的实验模型,关于其生殖生物学的基本方面(尤其是精子发生)的信息很少。因此,本研究旨在表征雄性生殖细胞,估计精子发生的持续时间,并评估纯睾丸中所选干细胞基因的表达。与其他鱼类相似,我们的研究结果表明,在Jundiá精子发生过程中,生殖细胞核的数量显着减少,特别是从未分化的A型精子到晚期的B型精原细胞,从双倍戊烯到晚期的精子。使用S-阶段标记物,溴脱氧尿苷(BrdU),估计该物种中减数分裂和生精阶段的合并持续时间约为7天。与精子发生持续30至74天的哺乳动物相比,这被认为非常短。选定的干细胞基因在Jundiá睾丸中进行了部分测序和鉴定。表达分析显示,在未分化的A型精原细胞富集的细胞级分中,较高的plzf和pou5f3 mRNA水平较高。这些结果通过原位杂交进一步证实,该原位杂交显示在A型未分化精原细胞中plzf和pou5f3 mRNA的强信号。总而言之,这些信息将扩大我们对该物种生殖生物学的知识,有助于改善其生产和管理,并有助于生物技术应用,例如生殖细胞移植。

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