The radioprotective action of a preparation from Hippophae rhamnoides berries RH-3, already reported to render >80% survival against whole body 10 Gy gamma irradiation, was further investigated with respect to the testicular system. RH-3 was administered to mice 30 min before gamma irradiation (5 and 10 Gy) and histological parameters such as testis weight, sperm count, frequency of abnormal sperm, repopulation index, stem cell survival index and seminiferous tubular diameter were assessed on the 35th day. RH-3 administration partially countered radiation induced reduction in testis weight, sperm count, repopulation index and stem cell survival index (p < 0.01). The increase in the frequency of abnormal sperm (15.17 +/- 1.046%) caused by irradiation (5 Gy) was counteracted by pre-irradiation treatment with RH-3, which significantly decreased the level of abnormal spermatozoa to 7.99 +/- 0.918% (p < 0.001), i.e. 52% abnormalities in comparison with 5 Gy irradiated group. RH-3 treatment alone did not elicit any toxic or adverse effect on the process of spermatogenesis. The present study suggests that RH-3 treatment protected spermatogenesis by enhancing the spermatogonial proliferation, enhancing the stem cell survival and reducing sperm abnormalities. The presence of polyphenolic flavonoids and tannins in the extract and the radical scavenging activity might be responsible for the radioprotective action of RH-3.

译文

:关于睾丸系统,进一步研究了沙棘浆果RH-3制剂的辐射防护作用,该制剂已报道其对全身10 Gyγ射线的存活率> 80%。在γ射线照射(5 Gy和10 Gy)前30分钟向小鼠施用RH-3,并在35日评估组织学参数,如睾丸重量,精子计数,精子异常频率,再填充指数,干细胞存活指数和生精小管直径。日。 RH-3给药部分抵消了辐射引起的睾丸重量,精子数量,再填充指数和干细胞存活指数的降低(p <0.01)。辐射(5 Gy)引起的异常精子频率增加(15.17 /-1.046%)被RH-3的预辐射处理所抵消,这使异常精子的水平显着降低至7.99 /-0.918%(p <0.001),即与5 Gy照射组相比,异常率为52%。单独使用RH-3处理不会对精子发生过程产生任何毒性或不利影响。本研究表明,RH-3处理可通过增强精原细胞的增殖,增强干细胞存活率和减少精子异常来保护精子发生。提取物中多酚类黄酮和单宁的存在以及自由基清除活性可能是RH-3辐射防护作用的原因。

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