PURPOSE:To assess transepithelial stromal riboflavin absorption with an enhanced riboflavin solution (riboflavin 0.1%, 15% dextran T500 with trometamol (Tris-[hydroxymethyl]aminomethane) and sodium ethylenediaminetetraacetic acid by analyzing light-transmission properties of ex vivo rabbit corneas. SETTING:School of Optometry and Vision Sciences, Cardiff, Wales. DESIGN:Experimental study. METHODS:The enhanced riboflavin drops (Ricrolin TE) were applied every 3 minutes for 1 hour to 12 corneas (4 with intact epithelium, 4 with superficial scratches, 4 with 8.0 mm epithelial debridement). As a comparison, riboflavin drops without the enhancers (riboflavin 0.1%, 20% dextran T500) (normal riboflavin group) were applied to 12 corneas (4 with intact epithelium, 4 with superficial scratches, 4 with central epithelial debridement). A control group of 4 corneas with intact epithelium received balanced saline 0.9%. To assess enhanced riboflavin absorption, light-transmission spectra of the corneas were analyzed with a spectrophotometer. RESULTS:The spectra in corneas with intact epithelium in both riboflavin groups and in eyes with superficial scratches treated with normal riboflavin were similar to controls. Those with enhanced riboflavin and superficial scratches showed a homogeneous yellow discoloration of the cornea with a dip in light transmission between 400 and 490 nm, similar to that of the enhanced riboflavin solution. This was also seen, albeit of a greater magnitude, with complete epithelial removal, with eyes receiving enhanced riboflavin having a greater dip in transmission than eyes receiving normal riboflavin. CONCLUSIONS:Administration of enhanced riboflavin and superficial epithelial scratches allowed sufficient riboflavin stromal absorption to homogeneously alter the transmission spectra of rabbit corneas. This did not occur to the same extent with an intact epithelium or normal riboflavin with superficial scratches. FINANCIAL DISCLOSURE:No author has a financial or proprietary interest in any material or method mentioned.

译文

目的:通过分析离体兔角膜的透光特性,评估增强的核黄素溶液(核黄素0.1%,15%右旋糖酐T500和丁苯三酚(Tris-[[羟甲基]氨基甲烷))和乙二胺四乙酸钠对上皮间质核黄素的吸收。
地点:威尔士卡迪夫验光与视觉科学学院。
设计:实验研究。
方法:每3分钟将增强的核黄素滴剂(Ricrolin TE)应用于12个角膜(4个完整的上皮,4个表面刮擦,4个8.0 mm上皮清创术)。作为比较,将不使用增强剂的核黄素滴剂(核黄素0.1%,右旋糖酐T500)(正常核黄素组)应用于12个角膜(4个完整上皮,4个表面刮擦,4个中央上皮清创)。带有完整上皮的4个角膜对照组接受0.9%的平衡盐水。为了评估核黄素吸收的增强,用分光光度计分析了角膜的透光光谱。
结果:核黄素组中角膜完整上皮的光谱和正常核黄素治疗后表面划痕的眼睛的光谱与对照组相似。具有增强的核黄素溶液和表面刮痕的那些显示出角膜的均匀黄变色,并且具有在400至490nm之间的光透射率的下降,类似于增强的核黄素溶液。还可以看到这种情况,尽管幅度更大,完全去除了上皮,与接受正常核黄素的眼睛相比,接受增强核黄素的眼睛的透射率下降更大。
结论:施用增强的核黄素和浅表上皮擦伤可使核黄素基质吸收充分,从而均匀地改变兔角膜的透射光谱。完整的上皮细胞或正常的核黄素表面有划痕时,发生这种情况的程度不同。
财务披露:任何作者都不会对所提及的任何材料或方法具有财务或专有利益。

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