Cloud point extraction with cold column trapping (CPE-CCT) was used for the rapid preconcentration and UV-Vis spectroscopy of beta-carotene in fruit juice samples. A central composite design was employed to optimize parameters such as pH, incubation time, cloud point temperature and surfactant concentration. A detection limit of 0.01 mg/L of beta-carotene (3SB/m), a coefficient of determination of 0.998 and a linear range of 0.04-10 mg/L were obtained. The CPE-CCT method was confirmed in comparison with the corresponding direct HPLC standard method. A simple, portable and cost-effective device was also utilized. Owing to eliminating centrifugation, the conditions of CPE-CCT were more moderate and its sample handling easier compared to conventional CPE.

译文

:使用冷柱捕集(CPE-CCT)进行浊点萃取可用于果汁样品中β-胡萝卜素的快速预富集和UV-Vis光谱分析。采用中央复合设计来优化参数,例如pH,孵育时间,浊点温度和表面活性剂浓度。 β-胡萝卜素(3SB / m)的检出限为0.01 mg / L,测定系数为0.998,线性范围为0.04-10 mg / L。与相应的直接HPLC标准方法相比,确定了CPE-CCT方法。还使用了一种简单,便携式且具有成本效益的设备。由于消除了离心作用,与传统的CPE相比,CPE-CCT的条件更为温和且样品处理更为轻松。

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