Infrared spectra of 13 globular proteins have been obtained in the 1800-1480-cm-1 region for H2O solutions. A method for estimating protein secondary structure from the ir spectrum has been developed. The method can also be used for estimating polypeptide and fibrous protein conformation. For the globular and fibrous proteins and polypeptides analyzed, the correlation coefficients between the ir and x-ray estimates of ordered helix, disordered helix, ordered beta-structure, disordered beta-structure, turns, and remainder were 0.98, 0.80, 0.99, 0.87, 0.90, and 0.92 respectively.

译文

:在H2O溶液的1800-1480-cm-1区域中已获得13种球状蛋白质的红外光谱。已经开发了一种从红外光谱估计蛋白质二级结构的方法。该方法还可用于估计多肽和纤维蛋白构象。对于所分析的球状和纤维状蛋白质和多肽,有序螺旋,无序螺旋,有序β结构,无序β结构,无序β和剩余的ir和x射线估计值的相关系数分别为0.98、0.80、0.99、0.87 ,分别为0.90和0.92。

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