Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the transplanted FND-labeled pcMSCs in organs and tissues of the miniature pigs after intravenous administration. The method is applicable to single-cell imaging and quantitative tracking of human stem/progenitor cells in rodents and other animal models as well.

译文

细胞疗法是治疗人类疾病的一种有前途的策略。尽管将细胞首次用于治疗目的可追溯到19世纪,但仍缺乏通用且可靠的方法来研究各种动物模型中用于临床前评估的移植细胞的生物分布和相关药代动力学。在这里,我们提出了一个新平台,该平台使用白蛋白缀合的荧光纳米金刚石(FND)作为生物相容性和光稳定的标签,用于通过磁调制定量跟踪微型猪中人胎盘绒毛膜来源的间充质干细胞(pcMSC)。通过这种无背景检测技术和定时荧光成像,我们已经能够精确确定静脉注射后在小型猪的器官和组织中移植的FND标记的pcMSC的数量和位置。该方法还适用于啮齿动物和其他动物模型中人干/祖细胞的单细胞成像和定量追踪。

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