Automated visual tracking of cells from video microscopy has many important biomedical applications. In this paper, we track human monocyte cells in a fluorescent microscopic video using matching and linking of bipartite graphs. Tracking of cells over a pair of frames is modeled as a maximum cardinality minimum weight matching problem for a bipartite graph with a novel cost function. The tracking results are further refined using a rank-based filtering mechanism. Linking of cell trajectories over different frames are achieved through composition of bipartite matches. The proposed solution does not require any explicit motion model, is highly scalable, and, can effectively handle the entry and exit of cells. Our tracking accuracy of (97.97±0.94)% is superior than several existing methods [(95.66±2.39)%, (94.42±2.08)%, (81.22±5.75)%, (78.31±4.70)%] and is highly comparable (98.20±1.22)% to a recently published algorithm.

译文

从视频显微镜对细胞进行自动视觉跟踪具有许多重要的生物医学应用。在本文中,我们使用二分图的匹配和链接在荧光显微视频中跟踪人单核细胞。一对框架上的单元格跟踪被建模为具有新颖成本函数的二部图的最大基数最小权重匹配问题。使用基于等级的过滤机制进一步完善跟踪结果。通过二分匹配的组成,可以实现不同框架上细胞轨迹的链接。提出的解决方案不需要任何显式的运动模型,具有高度可伸缩性,并且可以有效地处理单元的进入和退出。我们的(97.97±0.94)%跟踪精度优于几种现有方法[(95.66±2.39)%,(94.42±2.08)%,(81.22±5.75)%,(78.31±4.70)%],并且具有很高的可比性( 98.20±1.22)%至最近发布的算法。

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