Atherosclerosis is a major cause of death in the Western World. It is known that Lipofundin 20% induces atherosclerotic lesions, whereas ozone at low doses has been satisfactorily used in the prevention of oxidative stress-associated pathologies, such as coronary artery diseases. The aim of the present work was to evaluate the effects of ozone therapy on Lipofundin-induced atherosclerotic lesions in New Zealand White rabbits. Ozone (1 mg), mixed with oxygen as passive carrier, was administered by rectal insufflation during 15 sessions in 5 weeks. Then, the animals were intravenously treated with 2 mL/kg of Lipofundin, daily during 8 days. Animals were euthanized and eosin and hematoxylin staining was used for aortic histopathological analysis. The biomarkers of oxidative stress and lipid profile in serum were determined by spectrophotometric techniques. The results demonstrated that ozone induced inhibitory effects on aortic lesions formation. On the other hand, a reduction of biomolecular damage and an increase of antioxidant systems were observed at the end of the experiment. The serum lipids profiles were not modified after only 1 cycle of ozone treatment. Our results reinforced the hypotheses that antioxidant effects induced by ozone in the context of atherosclerosis demonstrate the antiatherogenic properties of the gas in the experimental conditions of this study.

译文

动脉粥样硬化是西方世界的主要死亡原因。已知Lipofundin 20% 诱导动脉粥样硬化病变,而低剂量的臭氧已令人满意地用于预防与氧化应激相关的病变,例如冠状动脉疾病。本工作的目的是评估臭氧疗法对新西兰白兔Lipofundin诱导的动脉粥样硬化病变的影响。臭氧 (1 mg) 与氧气混合,作为被动载体,在5周内的15个疗程中通过直肠吹气给药。然后,在8天内每天用2 ml/kg的Lipofundin静脉内治疗动物。对动物进行安乐死,并使用曙红和苏木精染色进行主动脉组织病理学分析。通过分光光度法测定血清中氧化应激和脂质谱的生物标志物。结果表明,臭氧对主动脉病变的形成具有抑制作用。另一方面,在实验结束时观察到生物分子损伤的减少和抗氧化系统的增加。仅经过1个臭氧处理周期后,血清脂质谱没有改变。我们的结果强化了以下假设: 在动脉粥样硬化的背景下,臭氧诱导的抗氧化作用证明了在本研究的实验条件下气体的抗动脉粥样硬化特性。

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