To examine the concordance of two microbial genotoxicity short-term assays, 330 experimental results for the SOS chromotest using tester strain Escherichia coli PQ37 were compared with the results of the Salmonella/mammalian microsome mutagenicity assay with Salmonella typhimurium TA97, TA98, TA100, TA102, TA104, TA1535, TA1537 and/or TA1538. With respect to qualitative features, the concordance between SOS chromotest and Salmonella mutagenicity test results was 86.4% (sensitivity, 78.6%; specificity, 100%; chi 2 = 188.6). None of the non-mutagens (N = 120) were able to induce the SOS system. Additionally, 45 of the 210 S.typhimurium mutagens (21.5%) did not induce the SOS repair system. On closer examination, the majority of these 45 compounds (84%) were mutagens with activities between 0.001 and 10 rev/nmol. Even though the experimental protocols of both systems were not standardized, the correlation coefficient for the experimental results of the two test systems was 0.7 for the 330 chemicals. Except for aliphatic epoxides (r = 0.47), the mutagenicity/SOS induction correlations for congeneric data sets (polycyclic aromatic hydrocarbons, nitroarenes, nitroarenofurans, mycotins) were even better (r = 0.72-0.95). Additionally, computer automated structure evaluation (CASE) analyses of the nature of the structural determinants associated with each endpoint indicate extensive homologies. The data can be taken to indicate that the two phenomena reflect common mechanisms of action.

译文

为了检验两种微生物遗传毒性短期测定的一致性,将330例使用测试菌株Escherichia coli PQ37进行SOS色度测试的实验结果与采用鼠伤寒沙门氏菌TA97,TA98,TA100,TA102, TA104,TA1535,TA1537和/或TA1538。就定性特征而言,SOS色度测试与沙门氏菌诱变测试结果之间的一致性为86.4%(敏感性为78.6%;特异性为100%; chi 2 = 188.6)。所有非突变体(N = 120)均无法诱导SOS系统。此外,210个鼠伤寒沙门氏菌中有45个(21.5%)没有诱导SOS修复系统。经仔细检查,这45种化合物中的大多数(84%)是诱变剂,其活性在0.001和10 rev / nmol之间。即使两个系统的实验方案均未标准化,但对于330种化学品,两个测试系统的实验结果的相关系数均为0.7。除脂族环氧化物(r = 0.47)外,同类数据集(多环芳烃,硝基芳烃,硝基芳呋喃,霉菌毒素)的诱变性/ SOS诱导相关性甚至更好(r = 0.72-0.95)。此外,与每个端点关联的结构决定因素的性质的计算机自动结构评估(CASE)分析表明,存在广泛的同源性。可以得出的数据表明这两种现象反映了共同的作用机理。

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