The 6C RNA family is a class of small RNAs highly conserved in Actinobacteria, including the genera Mycobacterium, Streptomyces and Corynebacterium whose physiological function has not yet been elucidated. We found that strong transcription of the cgb_03605 gene, which encodes 6C RNA in C. glutamicum, was driven by the SigA- and SigB-dependent promoter Pcgb_03605. 6C RNA was detected at high level during exponential growth phase (180 to 240 molcules per cell) which even increased at the entry of the stationary phase. 6C RNA level did not decrease within 240 min after transcription had been stopped with rifampicin, which suggests high 6C RNA stability. The expression of cgb_03605 further increased approximately twofold in the presence of DNA-damaging mitomycin C (MMC) and nearly threefold in the absence of LexA. Deletion of the 6C RNA gene cgb_03605 resulted in a higher sensitivity of C. glutamicum toward MMC and UV radiation. These results indicate that 6C RNA is involved in the DNA damage response. Both 6C RNA level-dependent pausing of cell growth and branched cell morphology in response to MMC suggest that 6C RNA may also be involved in a control of cell division.

译文

:6C RNA家族是放线菌中高度保守的一类小RNA,包括尚未阐明其生理功能的分枝杆菌属,链霉菌属和棒状杆菌属。我们发现,由SigA和SigB依赖性启动子Pcgb_03605驱动cgb_03605基因的强转录,该基因在谷氨酸棒杆菌中编码6C RNA。在指数生长期(每个细胞180到240分子),高水平检测到6C RNA,甚至在固定相进入时也增加。用利福平终止转录后240分钟内6C RNA水平没有降低,这表明6C RNA的稳定性很高。在存在破坏DNA的丝裂霉素C(MMC)的情况下,cgb_03605的表达进一步增加了约两倍,而在不存在LexA的情况下,其表达则增加了近三倍。 6C RNA基因cgb_03605的删除导致谷氨酸棒杆菌对MMC和UV辐射的敏感性更高。这些结果表明6C RNA参与了DNA损伤反应。响应MMC的6C RNA水平依赖性细胞生长暂停和分支细胞形态均表明6C RNA也可能参与细胞分裂的控制。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录