Four nitroarenes were tested in two standard genotoxicity assay systems using three well-known bacterial tester strains. The results were as follows: 4-nitroquinoline l-oxide (4NQO) was positive in Quillardet and Hofnung's SOS chromotest using Escherichia coli strain PQ37 both in the presence and absence of microsomal (S9) supplements and in the Salmonella typhimurium umu tester strains NM2009 and NM3009, which express high levels of O-acetyltransferase (O-AT) and O-AT plus nitroreductase (NR) respectively. m-Nitrocinnamic acid (m-NCA) was weakly positive in strains NM2009 and NM3009, but negative in the SOS chromotest; m-dinitrobenzene (m-DNB) was weakly positive in strain NM2009, intermediate positive in strain NM3009, but negative in the SOS chromotest; 2,4-dinitrotoluene (2,4-DNT) was weakly positive in strain NM3009, but negative in strain NM2009 and in the SOS chromotest. However it still showed a dose-response relationship in strain NM2009. In view of these results, it is suggested that investigators planning to screen miscellaneous nitroarenes for their genotoxicity in the future should consider taking advantage of the increased sensitivity which is conferred on S. typhimurium strains NM2009 and NM3009 by virtue of their capacity to overexpress O-AT or O-AT and NR.

译文

在四个标准的遗传毒性测定系统中,使用三种著名的细菌测试菌株对四个硝基芳烃进行了测试。结果如下:在存在和不存在微粒体(S9)补充剂和鼠伤寒沙门氏菌UM2009测试株中,使用大肠杆菌PQ37菌株在Quillardet和Hofnung的SOS色度测试中,4-硝基喹啉L-氧化物(4NQO)呈阳性。 NM3009,分别表达高水平的O-乙酰基转移酶(O-AT)和O-AT加硝基还原酶(NR)。间硝基肉桂酸(m-NCA)在NM2009和NM3009菌株中呈弱阳性,但在SOS色度测试中呈阴性。间二硝基苯(m-DNB)在NM2009菌株中呈弱阳性,在NM3009菌株中呈中等阳性,但在SOS色度测试中呈阴性。 2,4-二硝基甲苯(2,4-DNT)在NM3009菌株中呈弱阳性,而在NM2009菌株和SOS色度试验中呈阴性。然而,它仍然显示出菌株NM2009中的剂量反应关系。鉴于这些结果,建议研究人员计划将来筛选其他硝基芳烃的遗传毒性,应考虑利用鼠伤寒沙门氏菌菌株NM2009和NM3009的敏感性增强,因为它们具有过表达O-的能力。 AT或O-AT和NR。

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