The genotoxic activity of 11 mycotoxins was investigated in Escherichia coli K 12. The induction of the SOS function sfi A whose level of expression is monitored by means of a sfi A::lac Z operon fusion was assayed by measuring the beta-galactosidase activity in the PQ 37 strain. Most of these fungal metabolites did not induce SOS response in this bacterial test. Only aflatoxicol, a reduced metabolite of aflatoxin B1 was well detected as an SOS inducer if metabolic activation was performed. Patulin, penicillic acid and viomellein are only weak inducing agents. The other fungal compounds tested failed to demonstrate a positive SOS inducing activity. Relationship between SOS chromotest, mutagenicity to Salmonella typhimurium and in vivo carcinogenicity was discussed.

译文

:在大肠杆菌K 12中研究了11种霉菌毒素的遗传毒性活性。通过测量β-半乳糖苷酶活性来测定SOS功能sfi A的诱导,其表达水平通过sfi A :: lac Z操纵子融合体进行监测在PQ 37株中。这些真菌代谢物中的大多数在此细菌测试中均未诱导SOS反应。如果进行了代谢激活,则只有黄曲霉酚(一种降低的黄曲霉毒素B1代谢产物)可以作为SOS诱导剂被很好地检测到。棒曲霉素,青霉酸和堇青素仅是弱诱导剂。测试的其他真菌化合物未能显示出积极的SOS诱导活性。讨论了SOS显色性,对鼠伤寒沙门氏菌的致突变性与体内致癌性之间的关系。

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