Polychromatic flow-cytometric assays were used to analyze paired intrahepatic and peripheral lymphocyte samples from 37 patients with chronic hepatitis C. Compared with peripheral cells, intrahepatic T cells were selectively enriched with CD45RO+ memory T cells but had a lower percentage of CD4+ T cells expressing the differentiation markers CD27 and CD28. The percentage of intrahepatic CD45RO+ and CD28+ T cells correlated with the degree of liver inflammation, which suggests that memory T cells at relatively early stages of differentiation are directly involved in liver inflammation. Despite their memory phenotype, intrahepatic T cells were defective in proliferation capability, produced less interferon- gamma in response to stimulation by T cell receptor, and contained less perforin but expressed higher levels of Fas and Fas ligand, compared with their counterparts in peripheral blood. The distinct characteristics of intrahepatic T cells suggest that they play an important role in the immunopathogenesis of chronic hepatitis C.

译文

:采用多色流式细胞术分析了37例慢性丙型肝炎患者的肝内和外周血淋巴细胞配对样本。与外周血细胞相比,肝内T细胞选择性富集了CD45RO记忆T细胞,但表达CD4 T细胞的百分比较低分化标记CD27和CD28。肝内CD45RO和CD28 T细胞的百分比与肝脏炎症程度相关,这表明处于分化早期的记忆T细胞直接参与肝脏炎症。尽管具有记忆表型,但与外周血中的肝细胞相比,肝内T细胞在T细胞受体的刺激下应答能力较弱,产生的干扰素γ较少,穿孔素含量较低,但表达的Fas和Fas配体水平较高。肝内T细胞的独特特征表明它们在慢性丙型肝炎的免疫发病机制中起着重要作用。

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