BACKGROUND:Net blotch caused by Pyrenophra teres f. teres is a major foliar disease of barley. Infection can result in significant yield losses of susceptible cultivars of up to 40%. Of the two forms of net blotch (P. teres f. teres and P. teres f. maculata), P. teres f. teres (net form of net blotch) is the dominant one in Russia. The goal of the current study was to identify genomic regions associated with seedling resistance to several pathotypes of the net form of net blotch in Siberian spring barley genotypes. For this, a genome-wide association study of a Siberian barley collection, genotyped with 50 K Illumina SNP-chip, was carried out.
RESULTS:Seedling resistance of 94 spring barley cultivars and lines to four Pyrenophora teres f. teres isolates (S10.2, K5.1, P3.4.0, and A2.6.0) was investigated. According to the Tekauz rating scale, 25, 21, 14, and 14% of genotypes were highly resistant, and 19, 8, 9, and 16% of genotypes were moderate-resistant to the isolates S10.2, K5.1, P3.4.0, and A2.6.0, respectively. Eleven genotypes (Alag-Erdene, Alan-Bulag, L-259/528, Kedr, Krymchak 55, Omsky golozyorny 2, Omsky 13709, Narymchanin, Pallidum 394, Severny and Viner) were resistant to all studied isolates. Nine additional cultivars (Aley, Barkhatny, Belogorsky, Bezenchuksky 2, Emelya, G-19980, Merit 57, Mestny Primorsky, Slavaynsky) were resistant to 3 of the 4 isolates. The phenotyping and genotyping data were analysed using several statistical models: GLM + Q, GLM + PCA, GLM + PCA + Q, and the MLM + kinship matrix. In total, 40 SNPs in seven genomic regions associated with net blotch resistance were revealed: the region on chromosome 1H between 57.3 and 62.8 cM associated with resistance to 2 isolates (to P3.4.0 at the significant and K5.1 at the suggestive levels), the region on chromosome 6H between 52.6 and 55.4 cM associated with resistance to 3 isolates (to P3.4.0 at the significant and K5.1 and S10.2 at the suggestive levels), three isolate-specific significant regions (P3.4.0-specific regions on chromosome 2H between 71.0 and 74.1 cM and on chromosome 3H between 12.1 and 17.4 cM, and the A2.6.0-specific region on chromosome 3H between 50.9 and 54.8 cM), as well as two additional regions on chromosomes 2H (between 23.2 and 23.8 cM, resistant to S10.2) and 3 (between 135.6 and 137.5 cM resistant to K5.1) with suggestive SNPs, coinciding, however, with known net blotch resistance quantitative trait loci (QTLs) at the same regions.
CONCLUSIONS:Seven genomic regions on chromosomes 1H, 2H, 3H, and 6H associated with the resistance to four Pyrenophora teres f. teres isolates were identified in a genome-wide association study of a Siberian spring barley panel. One novel isolate-specific locus on chromosome 3 between 12.1 and 17.4 cM was revealed. Other regions identified in the current study coincided with previously known loci conferring resistance to net blotch. The significant SNPs revealed in the current study can be converted to convenient PCR markers for accelerated breeding of resistant barley cultivars.
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【SNPs associated with barley resistance to isolates of Pyrenophora teres f. teres.].
【SNP与大麦对拟南芥的分离株有抗性f。特雷斯。】
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DOI：10.1186/s12864-019-5623-3文章类型：杂志文章
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背景：由斑节杆菌引起的网斑病。麦汁是大麦的主要叶面疾病。感染可导致易感品种的产量损失高达40％。在两种形式的网斑病中（P. teres f。teres和P. teres f。maculata），P. teres f。 teres（网状斑点的净形式）在俄罗斯占主导地位。当前研究的目标是确定与幼苗对西伯利亚春大麦基因型净斑点净形态的几种病态抗性相关的基因组区域。为此，对西伯利亚大麦收集品进行了全基因组关联研究，并用50 K Illumina SNP芯片进行了基因分型。
结果：94个春大麦品种和品系对4个夏梨的幼苗的抗性。研究了分离株（S10.2，K5.1，P3.4.0和A2.6.0）。根据Tekauz评分量表，有25％，21％，14％和14％的基因型对分离株S10.2，K5.1，P3有中等抵抗力，有19％，8％，9％和16％的基因型对分离株S10.2，K5.1，P3有中等抵抗力.4.0和A2.6.0。 11种基因型（Alag-Erdene，Alan-Bulag，L-259 / 528，Kedr，Krymchak 55，Omsky golozyorny 2，Omsky 13709，Narymchanin，Pallidum 394，Severny和Viner）均具有抗性。另外9个品种（Aley，Barkhatny，Belogorsky，Bezenchuksky 2，Emelya，G-19980，Merit 57，Mestny Primorsky，Slavaynsky）对4个分离株中的3个有抗性。使用几种统计模型对表型和基因型数据进行了分析：GLM Q，GLM PCA，GLM PCA Q和MLM亲属矩阵。总共揭示了七个与净斑点抗性相关的基因组区域中的40个SNP：1H染色体上介于57.3和62.8 cM之间的区域与对2种分离物的抗性相关（显着性水平为P3.4.0，暗示水平为K5.1）。 ，即6H染色体上介于52.6和55.4 cM之间的区域，与对3个分离株的抵抗力相关（显着水平为P3.4.0，在提示水平为K5.1和S10.2），三个分离株特异性显着区域（P3.4.0- 2H染色体上的特定区域介于71.0和74.1 cM之间，3H染色体上的特定区域位于12.1和17.4 cM之间，3H染色体上的A2.6.0特定区域在50.9和54.8 cM之间），以及2H染色体上的两个附加区域（23.2之间）和23.8？cM，对S10.2有抵抗力； 3（对K5.1有抵抗力的135.6和137.5？cM之间）具有提示性SNP，但与此同时，在相同区域还具有已知的净斑点抗性定量性状基因座（QTL）。
结论：1H，2H，3H和6H染色体上的七个基因组区域与对四个Pyrenophora teres f的抗性有关。在西伯利亚春季大麦研究小组的全基因组关联研究中鉴定了这些分离株。揭示了一种新的分离物特异性位点，位于3号染色体上，介于12.1和17.4 cM之间。在本研究中鉴定出的其他区域与先前已知的赋予对净斑点的抗性的基因座相吻合。当前研究中揭示的重要SNP可以转化为方便的PCR标记，以加快抗性大麦品种的育种。
结果：94个春大麦品种和品系对4个夏梨的幼苗的抗性。研究了分离株（S10.2，K5.1，P3.4.0和A2.6.0）。根据Tekauz评分量表，有25％，21％，14％和14％的基因型对分离株S10.2，K5.1，P3有中等抵抗力，有19％，8％，9％和16％的基因型对分离株S10.2，K5.1，P3有中等抵抗力.4.0和A2.6.0。 11种基因型（Alag-Erdene，Alan-Bulag，L-259 / 528，Kedr，Krymchak 55，Omsky golozyorny 2，Omsky 13709，Narymchanin，Pallidum 394，Severny和Viner）均具有抗性。另外9个品种（Aley，Barkhatny，Belogorsky，Bezenchuksky 2，Emelya，G-19980，Merit 57，Mestny Primorsky，Slavaynsky）对4个分离株中的3个有抗性。使用几种统计模型对表型和基因型数据进行了分析：GLM Q，GLM PCA，GLM PCA Q和MLM亲属矩阵。总共揭示了七个与净斑点抗性相关的基因组区域中的40个SNP：1H染色体上介于57.3和62.8 cM之间的区域与对2种分离物的抗性相关（显着性水平为P3.4.0，暗示水平为K5.1）。 ，即6H染色体上介于52.6和55.4 cM之间的区域，与对3个分离株的抵抗力相关（显着水平为P3.4.0，在提示水平为K5.1和S10.2），三个分离株特异性显着区域（P3.4.0- 2H染色体上的特定区域介于71.0和74.1 cM之间，3H染色体上的特定区域位于12.1和17.4 cM之间，3H染色体上的A2.6.0特定区域在50.9和54.8 cM之间），以及2H染色体上的两个附加区域（23.2之间）和23.8？cM，对S10.2有抵抗力； 3（对K5.1有抵抗力的135.6和137.5？cM之间）具有提示性SNP，但与此同时，在相同区域还具有已知的净斑点抗性定量性状基因座（QTL）。
结论：1H，2H，3H和6H染色体上的七个基因组区域与对四个Pyrenophora teres f的抗性有关。在西伯利亚春季大麦研究小组的全基因组关联研究中鉴定了这些分离株。揭示了一种新的分离物特异性位点，位于3号染色体上，介于12.1和17.4 cM之间。在本研究中鉴定出的其他区域与先前已知的赋予对净斑点的抗性的基因座相吻合。当前研究中揭示的重要SNP可以转化为方便的PCR标记，以加快抗性大麦品种的育种。
