Peroxisome-proliferator-activated receptor gamma (PPARγ) is a key transcription factor that controls adipocyte differentiation and energy in mammals. Therefore, PPARγ is a potential factor influencing animal growth traits. This study primarily evaluates PPARγ as candidate gene for growth traits of cattle and identifies potential molecular marker for cattle breeding. Per previous studies, PPARγ mRNA was mainly expressed at extremely high levels in adipose tissues as shown by quantitative real-time polymerase chain reaction analysis. Three novel SNPs of the bovine PPARγ gene were identified in 514 individuals from six Chinese cattle breeds: SNP1 (AC_000179.1 g.57386668 C > G) in intron 2 and SNP2 (AC_000179.1 g.57431964 C > T) and SNP3 (AC_000179.1 g.57431994 T > C) in exon 7. The present study also investigated genetic characteristics of these SNP loci in six populations. Association analysis showed that SNP1 and SNP3 loci significantly affect weaning growth traits, especially body weight of Nanyang cattle. These results revealed that SNP1 and SNP3 are potential molecular markers for cattle breeding.

译文

:过氧化物酶体增殖物激活受体γ(PPARγ)是控制哺乳动物脂肪细胞分化和能量的关键转录因子。因此,PPARγ是影响动物生长性状的潜在因素。这项研究主要评估PPARγ作为牛生长特性的候选基因,并确定牛育种的潜在分子标记。根据先前的研究,如定量实时聚合酶链反应分析所示,PPARγmRNA主要在脂肪组织中高水平表达。在来自六个中国牛品种的514个个体中鉴定出三个牛PPARγ基因的新SNP:内含子2中的SNP1(AC_000179.1 g.57386668 C> G)和SNP2(AC_000179.1 g.57431964 C> T)和SNP3( AC_000179.1 g.57431994 T> C)外显子7。本研究还调查了这6个种群中这些SNP基因座的遗传特征。关联分析表明,SNP1和SNP3基因座显着影响断奶仔猪的生长性状,尤其是南阳牛的体重。这些结果表明,SNP1和SNP3是牛育种的潜在分子标记。

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