A cDNA clone (Tgzy85d11.r1) obtained from the Toxoplasma Expressed Sequence Tag project was chosen due to its homology with proteins of the heat shock 90 family. The cDNA encodes 137 amino acids of the C-terminal portion of the Toxoplasma Hsp90 protein (TgHsp90). Serum samples obtained from orally infected BALB/c and C57BL/6 mice showed reactivity against a recombinant TgHsp90 (rTgHsp90) after 8 weeks postinfection. Isotype analysis showed an anti-rTgHsp90 IgG2a/IgG3 response in infected BALB/c and anti-rTgHsp90 IgG1/IgG2a/IgG2b response in infected C57BL/6 mice. Serum samples from individuals chronically and putative acutely infected with T. gondii showed a similar anti-rTgHsp90 IgG response. Our work identifies TgHsp90 as a novel parasite antigen that seems to elicit a higher relation of anti-TgHsp90/anti-T. gondii IgGs during chronic infection in comparison with the acute stage.

译文

:选择从弓形虫表达序列标签项目获得的cDNA克隆(Tgzy85d11.r1),因为它与热休克90家族蛋白同源。 cDNA编码弓形虫Hsp90蛋白(TgHsp90)C端部分的137个氨基酸。从口腔感染的BALB / c和C57BL / 6小鼠获得的血清样品在感染后8周后显示出对重组TgHsp90(rTgHsp90)的反应性。同型分析表明,在感染的BALB / c中存在抗rTgHsp90 IgG2a / IgG3反应,在感染的C57BL / 6小鼠中具有抗rTgHsp90 IgG1 / IgG2a / IgG2b反应。弓形虫被慢性和假定急性感染的个体的血清样品显示出相似的抗rTgHsp90 IgG反应。我们的工作将TgHsp90鉴定为一种新型的寄生虫抗原,似乎引起了更高的抗TgHsp90 / anti-T关系。与急性期相比,在慢性感染期间感染了刚地IgG。

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