By screening a pigeon genomic DNA library, we isolated a recombinant phage clone containing the alpha(A)-globin gene. The DNA sequence of the approximately 6kbp-long insert fragment of the phage clone was determined. The sequence suggested the existence of pigeon alpha(D)-globin gene located 3.1 kbp upstream from the alpha(A)-globin gene. The expression of the alpha(D)-globin in late embryo was also shown by the N-terminal amino-acid sequence of the intact globin chain. These results show that two adult alpha-globin genes, alpha(A) and alpha(D), exist in the pigeon genome, and the alpha(D)-globin is expressed at the late embryo stage. The stage-specific expression suggests the existence of regulatory elements and factors interacting to inhibit transcription at the adult stage.

译文

:通过筛选鸽子基因组DNA文库,我们分离出了包含alpha(A)-globin基因的重组噬菌体克隆。确定了噬菌体克隆的约6kbp长的插入片段的DNA序列。该序列表明存在位于α(A)-球蛋白基因上游3.1 kbp处的鸽子α(D)-球蛋白基因。完整的球蛋白链的N-末端氨基酸序列也显示了晚期胚胎中α(D)-球蛋白的表达。这些结果表明,鸽子基因组中存在两个成年的α-球蛋白基因α(A)和α(D),并且α(D)-球蛋白在胚胎后期表达。该阶段特异性表达表明在成年阶段存在相互作用的调节元件和因子以抑制转录。

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