We used the multilocus sequence typing (MLST) method to evaluate the genetic diversity of 46 Campylobacter jejuni isolates from chickens and to determine the link between quinolone resistance and sequence type (ST). There were a total of 16 ST genotypes, and the majority of them belonged to seven clonal complexes previously identified by using isolates from human disease. The ST-353 complex was the most common complex, whereas the ST-21, ST-42, ST-52, and ST-257 complexes were less well represented. The resistance phenotype varied for each ST, and the Thr-86-Ile substitution in the GyrA protein was the predominant mechanism of resistance to quinolone. Nine of the 14 isolates having the Thr-86-Ile substitution belonged to the ST-353 complex. MLST showed that the emergence of quinolone resistance is not related to the diffusion of a unique clone and that there is no link between ST genotype and quinolone resistance. Based on silent mutations, different variants of the gyrA gene were shown to exist for the same ST. These data provide useful information for understanding the epidemiology of C. jejuni in Senegal.

译文

:我们使用多基因座序列分型(MLST)方法评估了鸡中46株空肠弯曲杆菌分离株的遗传多样性,并确定了喹诺酮耐药性与序列类型(ST)之间的联系。一共有16种ST基因型,其中大多数属于先前使用人类疾病分离株鉴定出的7种克隆复合体。 ST-353复合物是最常见的复合物,而ST-21,ST-42,ST-52和ST-257复合物的代表性较差。每个ST的抗性表型各不相同,GyrA蛋白中的Thr-86-Ile取代是对喹诺酮抗性的主要机制。具有Thr-86-Ile取代的14个分离株中有9个属于ST-353复合体。 MLST表明,喹诺酮耐药性的出现与独特克隆的扩散无关,并且ST基因型与喹诺酮耐药性之间没有联系。基于沉默突变,表明对于同一ST存在gyrA基因的不同变体。这些数据为了解塞内加尔空肠弯曲杆菌的流行病学提供了有用的信息。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录