With oligonucleotides based on the amino-terminal and internal amino-acid sequences of a xylanase, two xylanase genes, cgxA and cgxB, were isolated and sequenced from Chaetomium gracile wild and mutant strains. Each gene isolated from both strains was essentially the same as far as nucleotide sequences were compared. The mature CgXA and CgXB xylanases comprise 189 and 211 amino acids, respectively, and share 68.5% homology. The CgXA was found to be the major enzyme in the mutant strain. Comparison of these amino-acid sequences with xylanase sequences from other origins showed that they have a high degree of identity to the family G xylanases. The cgxA and cgxB genes were introduced into Aspergillus nidulans and found to be expressed with their own promoters.

译文

使用基于木聚糖酶的氨基末端和内部氨基酸序列的寡核苷酸,从毛Chaetomium gracile野生和突变菌株中分离并测序了两个木聚糖酶基因cgxA和cgxB。就比较核苷酸序列而言,从两个菌株中分离出的每个基因基本相同。成熟的CgXA和CgXB木聚糖酶分别包含189和211个氨基酸,并且具有68.5% 同源性。发现CgXA是突变菌株中的主要酶。这些氨基酸序列与其他来源的木聚糖酶序列的比较表明,它们与G族木聚糖酶具有高度的同一性。将cgxA和cgxB基因引入到构巢曲霉中,并发现它们与自己的启动子一起表达。

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