The utility of the Aspergillus fumigatus cellobiohydrolase cbhB promoter for controlled gene expression has been investigated. cbhB message was present at high levels in the presence of carboxymethylcellulose and undetected in the presence of glucose. A reporter construct using the cbhB promoter showed similar behaviour and gave lower message levels than the Aspergillus nidulans alcA promoter under repressing conditions. An RNAi construct driven by the cbhB promoter was used to down-regulate the alb1 gene; transformants showed low alb1 message levels and a loss-of-function phenotype with carboxymethylcellulose, while both wild-type message levels and phenotype were seen with glucose. The cbhB promoter is therefore tightly controlled and can be exploited for the study of A. fumigatus.

译文

研究了烟曲霉纤维二糖水解酶cbhB启动子在控制基因表达中的效用。cbhB信息在羧甲基纤维素存在下以高水平存在,而在葡萄糖存在下未检测到。在抑制条件下,使用cbhB启动子的报告构建体表现出相似的行为,并且给出的信息水平低于构巢曲霉alcA启动子。由cbhB启动子驱动的RNAi构建体用于下调alb1基因; 转化子显示出较低的alb1信息水平和羧甲基纤维素的功能丧失表型,而葡萄糖则观察到野生型信息水平和表型。因此,cbhB启动子受到严格控制,可用于烟曲霉的研究。

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