OBJECTIVE:Febrile seizures (FS) are the most common convulsions in childhood. Interleukin-1beta (IL-1β) is proposed to play an important role in the development of FS, from in vitro data and data from peripheral blood samples. IL-1β secretion is needed for activation of the NLR family, pyrin-domain containing 3(NLRP3) inflammasome. However, whether NLRP3 play a role in the development of FS remains unknown. This study aimed to investigate the role of NLRP3 in FS. METHODS:Thirty-two FS cases and twenty-two matched controls were included in this study. Control samples were collected from children with febrile illness without seizures. We detected their levels of IL-1β and NLRP3 by Enzyme linked immunosorbent assay and Western blot, respectively. RESULTS:Serum IL-1β levels weresignificantlyhigher in FS patients (Median = 301.64 pg/ml) than in fever only controls (Median = 159.48 pg/ml) (P < 0.05). Additionally, NLRP3 protein levels of peripheral blood mononuclear cells (PBMC) were significantly higher in typical FS than in fever only controls (P < 0.05). Moreover, serum levels of IL-1β were significantly correlated with levels of NLRP3 protein (r = 0.787, P < 0.001). CONCLUSIONS:In this study, our results firstly indicated that NLRP3 protein was significantly up-regulated in the typical FS children compared in fever only controls. Increased NLRP3 can mediate IL-1β secretion that is responsible for the occurrence of FS.