Plant dehydrin proteins (DHNs) are known to be important for environmental stress tolerance and are involved in various developmental processes. Two full-length cDNAs JcDHN-1 and JcDHN-2 encoding two dehydrins from Jatropha curcas seeds were identified and characterized. JcDHN-1 is 764 bp long and contains an open reading frame of 528 bp. The deduced JcDHN-1 protein has 175 a.a. residues that form a 19.3-kDa polypeptide with a predicted isoelectric point (pI) of 6.41. JcDHN-2 is 855 bp long and contains an open reading frame of 441 bp. The deduced JcDHN-2 protein has 156 a.a. residues that form a 17.1-kDa polypeptide with a predicted pI of 7.09. JcDHN-1 is classified as type Y₃SK₂ and JcDHN-2 is classified as type Y₂SK₂ according to the YSK shorthand for structural classification of dehydrins. Homology analysis indicates that both JcDHN-1 and JcDHN-2 share identity with DHNs of other plants. Analysis of the conserved domain revealed that JcDHN-2 has glycoside hydrolase GH20 super-family activity. Quantitative real time PCR analysis for JcDHN-1 and JcDHN-2 expression during seed development showed increasing gene expression of both their transcript levels along with the natural dehydration process during seed development. A sharp increase in JcDHN-2 transcript level occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. These results indicate that both JcDHNs have the potential to play a role in cell protection during dehydration occurring naturally during jatropha orthodox seed development.

译文

植物脱水素蛋白(DHNs)对于环境胁迫耐受性很重要,并参与各种发育过程。鉴定并鉴定了编码来自麻风树种子的两个脱水蛋白的两个全长cDNA JcDHN-1和JcDHN-2。 JcDHN-1长764 bp,包含一个528 bp的开放阅读框。推导的JcDHN-1蛋白具有175 a.a.形成19.3 kDa多肽的残基,其预测的等电点(pI)为6.41。 JcDHN-2长855 bp,包含一个441 bp的开放阅读框。推导的JcDHN-2蛋白具有156 a.a.形成17.1kDa多肽的残基,其预测pI为7.09。根据YSK简写为脱水醇的结构分类,JcDHN-1被归类为Y₃SK2,JcDHN-2被归类为Y 2SK 2。同源性分析表明,JcDHN-1和JcDHN-2与其他植物的DHN共享同一性。对保守结构域的分析显示JcDHN-2具有糖苷水解酶GH20超家族活性。种子发育过程中JcDHN-1和JcDHN-2表达的实时定量PCR分析显示,它们的转录水平的基因表达都随着种子发育过程中的自然脱水过程而增加。水分含量从成熟种子的42%下降到干燥种子的12%时,JcDHN-2转录水平急剧增加。这些结果表明,两种JcDHN都可能在麻风树正统种子发育过程中自然发生的脱水过程中发挥细胞保护作用。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录