White clover widely cultivated in China is one of the most important perennial leguminous forages in temperate and subtropical regions. There is a large quantity of white clover seeds imported into China each year for demands of high-quality grass seeds. Seedborne diseases may cause significant economic losses. DNA sequencing technologies allow for the direct estimation of microbial community diversity, avoiding culture-based biases. Therefore, we used 16S rRNA gene sequencing to investigate the bacterial communities in white clover seeds collected from four different countries. The results showed that a total of 484,715 clean reads were obtained for further subsequent analysis. In total, 341, 340, 382, and 297 operational taxonomic units were obtained at 3% distance cutoff in DB, MB, TB, and XB samples, respectively. The richness indexes revealed that TB sample from Argentina had the highest bacterial richness in four samples. Our results demonstrated that Proteobacteria was the dominant phyla in MB, TB, and XB; however, Bacteroidetes was the dominant phyla in DB. The dominant genus of DB was Prevotella (11.9%), while Sphingomonas was the major genus of MB (46.9%), TB (55.08%), and XB (47.2%) samples. These results provide useful information for seedborne diseases and transmission of bacteria from seed to seedling.

译文

:在中国广泛种植的白三叶草是温带和亚热带地区最重要的多年生豆科牧草之一。每年有大量的白三叶草种子进口到中国,以满足对优质草种子的需求。种子传播的疾病可能会造成重大的经济损失。 DNA测序技术可直接估算微生物群落的多样性,避免了基于培养的偏倚。因此,我们使用16S rRNA基因测序来研究从四个不同国家收集的白三叶草种子中的细菌群落。结果显示,总共获得了484,715次干净读取,以进行进一步的后续分析。在DB,MB,TB和XB样本中,以3%的距离截止,总共获得了341、340、382和297个操作分类单位。丰富度指数表明,来自阿根廷的结核病样品在四个样品中细菌含量最高。我们的研究结果表明,变形杆菌是MB,TB和XB中的显性门。然而,拟杆菌属是DB中的主要门。 DB的主要属是普雷沃氏菌(11.9%),鞘氨醇单胞菌是MB(46.9%),TB(55.08%)和XB(47.2%)样品的主要属。这些结果为种子传播疾病和细菌从种子传播到幼苗提供了有用的信息。

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