The larvicidal activity of crude petroleum ether, toluene, n-butanol, ethyl acetate, acetone, and methanol extracts of the seeds of Clausena lansium was assayed for their toxicities against the early fourth instar larvae of Aedes albopictus. The larval mortality was observed after 24-h exposure. The LC(50) value of petroleum ether extract was 22.99 ppm, showing the best larvicidal activity among all six solvent extracts. A cinnamon amide compound lansiumamide B (N-methyl-N-cis-styrylcinnamamide) was isolated from the petroleum ether extract by column chromatographic method, which exhibited a strong larvicidal activity against the early fourth instar larvae of A. albopictus with LC(50) and LC(90) values of 0.45 and 2.19 ppm, respectively. The structure was elucidated by (1)H NMR, (13)C NMR spectral data. The larvicidal activity against mosquito of lansiumamide B from the seed of C. lansium was evaluated for the first time.

译文

:测定了Clausena lansium种子的粗石油醚,甲苯,正丁醇,乙酸乙酯,丙酮和甲醇提取物的杀幼虫活性,对白纹伊蚊的早期四龄幼虫具有毒性。暴露24小时后观察到幼虫死亡率。石油醚提取物的LC(50)值为22.99 ppm,在所有六种溶剂提取物中显示出最佳的杀幼虫活性。通过柱色谱法从石油醚提取物中分离出肉桂酰胺化合物镧酰胺B(N-甲基-N-顺式-苯乙烯基肉桂酰胺),该化合物对LC.50的白斑拟南芥幼虫具有很强的杀幼虫活性。和LC(90)值分别为0.45和2.19 ppm。通过(1)H NMR,(13)C NMR光谱数据阐明结构。首次评价了来自镧梭菌种子的镧酰胺B对蚊子的杀幼虫活性。

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