A sensitive liquid chromatography/electrospray ionization tandem mass spectrometric (LC/ESI-MS/MS) method was developed for the identification and quantification of two antihepatotoxic coumarinolignoids cleomiscosin A and cleomiscosin B in different extracts of the seeds of Cleome viscosa. The separation of cleomiscosin A and cleomiscosin B was achieved on an RP(18) column using a solvent system consisting of a mixture of acetonitrile-methanol (1:2, v/v) and acetonitrile-water-formic acid (5:95:0.3, v/v) as a mobile phase in a gradient elution mode. A multiple-reaction monitoring (MRM) method was developed for quantification of cleomiscosin A and cleomiscosin B in the seed extracts of Cleome viscosa. On the basis of signal-to-noise ratio of 3, the limit of detection in MRM mode for cleomiscosin A and cleomiscosin B were 1.0 and 4.0 ng/mL respectively. The method was validated in terms of linearity, accuracy and precision for 6 days. The method developed was found to be useful for identification and quantification of cleomiscosin A and cleomiscosin B in the different extracts of the seeds of Cleome viscosa.

译文

:开发了一种灵敏的液相色谱/电喷雾串联质谱法(LC / ESI-MS / MS),用于鉴定和定量粘胶粘菌种子不同提取物中的两种抗肝毒香豆素类固醇A和粘菌B。使用包含乙腈-甲醇(1:2,v / v)和乙腈-水-甲酸(5:95: 0.3,v / v)作为梯度洗脱模式下的流动相。建立了一种多重反应监测(MRM)方法,用于定量测定粘粘捕捞蝇种子提取物中的粘菌素A和粘菌素B。在信噪比为3的基础上,MRM模式下的粘菌素A和粘菌素B的检出限分别为1.0 ng / mL和4.0 ng / mL。该方法经过6天的线性,准确度和精密度验证。已发现开发的方法可用于鉴定和定量粘菌油料种子的不同提取物中的粘菌素A和粘菌素B。

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