The dynamic activities of mitochondria and lysosomes, which play important roles in maintaining cellular homeostasis, were observed without labeling by using highly sensitive photothermal (PT) microscopy. This imaging modality allows for the direct observation of cellular organelles that contain endogenous chromophores, with high temporal and spatial resolution. We identified mitochondria and lysosomes inside living mammalian cells via simultaneous dual-color imaging. Moreover, dynamic imaging revealed that the lysosomes make contact with mitochondria and move between sites within the dynamic mitochondrial network. Since mitochondrial and lysosomal functions are intricately connected, PT microscopy should provide in-depth understanding of cellular functions associated with mitochondria-lysosome communication as well as insights into various human diseases caused by dysfunction of these organelles.

译文

:通过使用高灵敏度的光热(PT)显微镜,无需标记即可观察到线粒体和溶酶体的动态活动,它们在维持细胞稳态中起着重要作用。这种成像方式可以以高的时间和空间分辨率直接观察包含内生发色团的细胞器。我们通过同步双色成像鉴定了活的哺乳动物细胞内的线粒体和溶酶体。此外,动态成像显示溶酶体与线粒体接触并在动态线粒体网络内的位点之间移动。由于线粒体功能和溶酶体功能错综复杂地联系在一起,因此,PT显微镜应能深入了解与线粒体-溶酶体通讯相关的细胞功能,并深入了解由这些细胞器功能障碍引起的各种人类疾病。

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