BACKGROUND:Compromised sexual health is a major rehabilitative barrier for men with lower-spinal cord injury (SCI). Although studies have revealed decreased sperm motility, the quantitative biochemical changes that underlie the infertility mechanism remain poorly understood. METHODS:We employed a nontargeted approach combining 800 MHz hydrogen nuclear magnetic resonance ((1)H NMR) spectroscopy and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) with pattern recognition methods to analyze seminal fluid metabolite profiles in 10 men with and 8 without SCI above thoracic vertebra 10 (T10). RESULTS:The metabolic phenotype for SCI could be predicted from the (1)H NMR data. The median concentration of uridine in fertile controls was 1.55 mmol/L (range 1.0-5.0 mmol/L), but was undetectable by both NMR and MS in all but 2 individuals from the SCI group, one who later fathered a child without assisted fertility techniques. CONCLUSIONS:We hypothesize that uridine is likely to be an essential precursor to metabolites required for capacitation and is a potential marker for the prognosis of post-SCI functional fertility recovery. We derived the term "seminal oligouridinosis" to describe this newly identified condition.

译文

背景:性健康受损是下脊髓脊髓损伤(SCI)男性的主要康复障碍。尽管研究表明精子活力下降,但不孕机制背后的定量生化变化仍知之甚少。
方法:我们采用了一种非靶向方法,结合了800 MHz氢核磁共振波谱((1)H NMR)和超高效液相色谱-质谱(UPLC-MS)以及模式识别方法,以分析10名男性的精液代谢产物概况。 8在胸椎10(T10)上方没有SCI。
结果:可以通过(1)H NMR数据预测SCI的代谢表型。受精对照中尿苷的中位浓度为1.55 mmol / L(范围为1.0-5.0 mmol / L),但除了SCI组的2名患者外,其他所有患者均无法通过NMR和MS检测到,该患者后来生下了一个没有辅助生育能力的孩子技术。
结论:我们假设尿苷可能是获能所需代谢产物的重要前体,并且是SCI后功能生育能力恢复的预后指标。我们衍生出术语“先天性寡尿尿症”来描述这种新发现的疾病。

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