The type III secretion system (T3SS) of gram-negative bacteria involves dedicated protein translocation machinery that directly injects proteins into target cells. Pathogenic bacteria already benefit from this unique system. The successful functional cloning of this useful tool into non-pathogenic bacteria would help establish novel clinical and basic biotechnology strategies in areas such as vaccine administration, the development of screening systems for anti-T3SS drugs and the target-specific delivery of bioactive compounds. In this study, we successfully cloned the Vibrio parahaemolyticus T3SS1 genetic locus into a non-pathogenic Escherichia coli K-12 strain. Assays performed here revealed that the T3SS1 cloned into the E. coli K-12 strain has the ability to translocate V. parahaemolyticus T3SS1 secreted proteins. Importantly, we also observed this system to allow the E. coli K-12 strain to inject foreign protein, as well as the V. parahaemolyticus T3SS effector, into cultured cells. These results demonstrate a prospective useful tool with experimental and therapeutic applications.

译文

:革兰氏阴性细菌的III型分泌系统(T3SS)涉及专用的蛋白质转运机制,可直接将蛋白质注射到靶细胞中。致病细菌已经从这种独特的系统中受益。将该有用工具成功地功能克隆到非致病性细菌中,将有助于在疫苗管理,抗T3SS药物筛选系统的开发以及生物活性化合物的靶标特异性递送等领域建立新颖的临床和基础生物技术策略。在这项研究中,我们成功地将副溶血弧菌T3SS1遗传基因座克隆到了非致病性大肠杆菌K-12菌株中。此处进行的分析表明,克隆到大肠杆菌K-12菌株中的T3SS1具有转移副溶血弧菌T3SS1分泌蛋白的能力。重要的是,我们还观察到该系统允许大肠杆菌K-12菌株向培养的细胞中注入外源蛋白以及副溶血弧菌T3SS效应子。这些结果证明了具有实验和治疗应用前景的有用工具。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录