Thirteen cultivars of hop (Humulus lupulus L.) were tested by reverse transcription-polymerase chain reaction (RT-PCR) for the presence of apple mosaic virus (ApMV). The virus was detected in various amounts in all tested cultivars. Control hop clones derived from tissue cultures, treated by thermotherapy and maintained in greenhouse were virus-free. The procedure for sample preparation and RT-PCR of ApMV is described in detail.

译文

通过逆转录-聚合酶链反应(RT-PCR)测试了13个酒花(Humulus lupulus L.)的苹果花叶病毒(ApMV)的存在。在所有测试品种中都检测到了各种数量的病毒。来自组织培养物的对照蛇麻草克隆,经热疗处理并保存在温室中,均不含病毒。详细描述了ApMV的样品制备和RT-PCR步骤。

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