The present experiments examined the effects of muscarinic cholinergic receptor blockade in the nucleus accumbens (NAC) and medial prefrontal cortex (MPC) on intravenous cocaine self-administration. Adult male Sprague-Dawley rats were implanted with chronic indwelling jugular catheters and guide cannulae stereotaxically aimed at the NAC or MPC. The rats were then given the opportunity to intravenously self-administer cocaine (0.8 mg/kg/infusion) during daily 2-h sessions. Intra-NAC microinjections of methyl-scopolamine (2, 4, 8, 16, and 32 microg/side) or vehicle did not affect either the number of lever presses made or infusions delivered. On the other hand, intra-MPC injections of scopolamine significantly increased responding, although there was only a trend for an increase in the number of cocaine infusions. The effects of intra-MPC injections of scopolamine (8 and 16 microg/side) on locomotor activity were also evaluated. Intra-MPC injections of scopolamine (16 microg/side) produced significant increases in locomotor activity. However, these same microinjections decreased locomotor activity when the animals also received cocaine (15 mg/kg, i.p.). These results suggest that cholinergic neurotransmission at muscarinic receptors in the MPC is involved in regulating cocaine-maintained responding.

译文

:本实验研究了伏伏核(NAC)和内侧前额叶皮层(MPC)中毒蕈碱胆碱能受体阻滞对静脉注射可卡因自我给药的影响。将成年雄性Sprague-Dawley大鼠植入慢性留置颈静脉导管,并立体定向引导套管对准NAC或MPC。然后,在每天的2小时疗程中,给大鼠提供了静脉内自行施用可卡因(0.8毫克/千克/输注)的机会。甲基东sco碱(2、4、8、16和32微克/面)或媒介物的NAC内微注射既不影响杠杆按压的次数,也不影响输注量。另一方面,MPC内注射东pol碱的反应显着增强,尽管可卡因输注次数仅有增加的趋势。还评估了东pol碱MPC内注射东pol碱(每侧8和16微克)对运动活性的影响。东pol碱的MPC内注射(16微克/面)可显着提高运动能力。但是,当动物也接受可卡因(15 mg / kg,腹腔注射)时,这些相同的显微注射会降低运动能力。这些结果表明,MPC中毒蕈碱受体的胆碱能神经传递参与调节可卡因维持的应答。

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