Immune therapy have shown new and exciting perspectives for cancer treatment. Aim of our study was to evaluate toxicity and possible adverse effects from vaccination of patients with advanced colorectal cancer with autologous dendritic cells (DC) pulsed with lysate from a newly developed melanoma cell line, DDM-1.13. Six patients were enrolled in the phase I trial. Autologous DCs were generated in vitro from peripheral blood monocytes in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). DCs were pulsed with melanoma cell lysate from a cloned and selected melanoma cell line enriched in expression of MAGE-A antigens and deficient in expression of melanoma differentiation antigens: tyrosinase, MART-1 and gp100. Vaccinations were administered intradermally on the proximal thigh with a total of five given vaccines at 2 weeks intervals. Each vaccine contained 3-5 x 10(6) DCs. Five of the six patients received all five vaccines. The treatment was well tolerated in all patients without any observed vaccine-correlated adverse effects. Treatment with this DC-based cancer vaccine proved safe and non-toxic.

译文

:免疫疗法已显示出令人兴奋的癌症治疗新观点。我们研究的目的是评估用新开发的黑素瘤细胞系DDM-1.13裂解物脉冲的自体树突状细胞(DC)对晚期结直肠癌患者进行疫苗接种的毒性和可能的​​不良反应。一期试验招募了六名患者。在粒细胞-巨噬细胞集落刺激因子(GM-CSF)和白介素-4(IL-4)的存在下,由外周血单核细胞体外产生自体DC。用来自克隆和选择的黑色素瘤细胞系的黑色素瘤细胞裂解液对DC进行脉冲处理,该黑色素瘤细胞系富含MAGE-A抗原的表达,而黑色素瘤分化抗原(酪氨酸酶,MART-1和gp100)的表达不足。在大腿近端皮内注射疫苗,每两周间隔注射五种给定的疫苗。每种疫苗均包含3-5 x 10(6)个DC。六名患者中有五名接受了全部五种疫苗的治疗。所有患者对治疗的耐受性良好,没有观察到疫苗相关的不良反应。用这种基于DC的癌症疫苗治疗证明是安全且无毒的。

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