A differentiation, based on morphological characters, between Stylonychia mytilus and Stylonychia lemnae is very difficult, especially for non-specialists. These two sibling species were considered as one species, S. mytilus, until detailed cytological and genetic studies could show the existence of two genetically isolated varieties. Further morphological and biochemical analyses verified the separation and finally in 1983 a new species S. lemnae was described. The examination of several isoenzymes revealed unambiguous differences in the banding pattern of isocitrate dehydrogenase (IDH) between these two species. Therefore, the IDH gene of 30 isolates of S. lemnae and S. mytilus coming from various regions all over the world were amplified and sequenced. The sequence analyses revealed intraspecific as well as interspecific substitutions, which were used for the development of species-specific PCR primers for both species. Application of these species-specific primer pairs now allows a very easy and clear identification of both sibling species.

译文

:在形态特征上,Mytilus和Stylonychia lemnae之间的区分是非常困难的,特别是对于非专业人士而言。直到详细的细胞学和遗传学研究表明存在两个遗传分离的变种之前,这两个兄弟姐妹种才被认为是一种S. mytilus。进一步的形态学和生化分析证实了分离,最后在1983年描述了一个新种S. lemnae。对几种同工酶的检查表明,这两个物种之间的异柠檬酸脱氢酶(IDH)的带谱模式无明显差异。因此,扩增并测序了来自世界各地的30株柠檬肉链球菌和枯草链球菌的IDH基因。序列分析揭示了种内和种间取代,这些被用于开发两个物种的物种特异性PCR引物。现在,这些物种特异性引物对的应用可以非常轻松,清晰地鉴定两个同胞物种。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录