Numerous studies have shown that immunostimulatory complexes containing Quil-A saponin and various antigens are effective in stimulating the immune response and can be used as vaccine preparations for animals and humans. However, Quil-A saponin possesses toxicity and haemolytic activity. In the present work, a saponin-containing preparation named "Glabilox" was isolated from the roots of a Glycyrrhiza glabra L. plant by high-performance liquid chromatography (HPLC). The results showed that Glabilox has no toxicity or haemolytic activity and can form stable immunostimulatory complexes. Subcutaneous immunization of mice with an immunostimulating complex containing Glabilox and H7N1 influenza virus antigens stimulated high levels of humoral and cellular immunity. Vaccination of chickens with the same immunostimulating complex protected 100% of the animals after experimental infection with a homologous virus. Comparative studies showed that the immunogenic and protective activity of immunostimulatory complexes containing Quil-A and immunostimulatory complexes containing Glabilox are comparable to each other. The results of these studies indicated that Glycyrrhiza glabra saponins show great promise as safe and effective adjuvants.

译文

:大量研究表明,含有Quil-A皂苷和各种抗原的免疫刺激复合物可有效刺激免疫反应,并可作为动物和人类的疫苗制剂。但是,Quil-A皂苷具有毒性和溶血活性。在本工作中,通过高效液相色谱法(HPLC)从甘草中的植物的根中分离出了一种名为“ Glabilox”的含皂苷的制剂。结果表明,Glabilox没有毒性或溶血活性,可以形成稳定的免疫刺激复合物。用含有Glabilox和H7N1流感病毒抗原的免疫刺激复合物对小鼠进行皮下免疫可刺激高水平的体液和细胞免疫。实验性感染同源病毒后,用相同的免疫刺激复合物对鸡进行疫苗接种可保护100%的动物。比较研究表明,包含Quil-A的免疫刺激复合物和包含Glabilox的免疫刺激复合物的免疫原性和保护活性彼此相当。这些研究结果表明甘草皂苷作为安全有效的佐剂具有广阔的前景。

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