To explore protective mechanism of Panax notoginseng saponins (PNS) on rat hemorrhagic shock model in recovery stage. 72 Wistar rats were selected and divided into control group, model group and PNS group with 24 rats in each group. 200 mg/kg PNS was injected intravenously at 60 min of hemorrhagic shock stage in PNS groups. Changes of endotoxin, MPO, IL-6, SOD, MDA and TNF α were observed at 30 and 120 min of recovery stage by ELISA; water content of lung and intestine was detected; HE staining was applied to observe morphological change of intestinal mucosa, kidney, liver and lung; western blot was used to detect intercellular adhesion molecule-1 (ICAM-1) level in lung tissue and intestine tissue. At 30 min and 120 min of recovery stage, MDA, MPO, endotoxin, TNF α and IL-6 levels significantly increased in model group compared with control group, however SOD level significantly decreased, the difference was statistically significant (P < 0.05); PNS dose-dependently decreased MDA, MPO, endotoxin, TNF α and IL-6 levels, and increased SOD level, which was statistically significant (P < 0.05); In results of water content detection, water content in lung tissue and intestine tissue was significantly higher than in control group, however, after being treated with PNS, the water content was significantly decreased; HE staining showed the morphologic change of lung tissue cells; Western blot showed that in lung tissue and intestine tissue, ICAM-1 level in model group was significantly higher than in control group, and it was lower in PNS group than in model group. PNS can increase SOD activity, decrease levels of MDA, endotoxin and MPO, decrease expression of TNF α and IL-6, and decrease water content in lung tissue and intestine tissue. Thus, PNS is protective to rat hemorrhagic shock model by anti oxidative stress and anti-inflammatory pathways, and ICAM-1 may play an important role in the mechanism.

译文

:探讨三七总皂甙(PNS)对大鼠失血性休克恢复期的保护机制。选择Wistar大鼠72只,分为对照组,模型组和PNS组,每组24只。 PNS组在失血性休克60分钟时静脉注射200 mg / kg PNS。 ELISA法在恢复期30和120分钟观察到内毒素,MPO,IL-6,SOD,MDA和TNFα的变化;检测肺和肠中的水分; HE染色观察肠黏膜,肾脏,肝脏和肺的形态变化。免疫印迹用于检测肺组织和肠组织中的细胞间粘附分子-1(ICAM-1)水平。恢复期30min和120min,模型组MDA,MPO,内毒素,TNFα和IL-6水平较对照组明显升高,但SOD水平明显降低,差异有统计学意义(P <0.05); PNS剂量依赖性地降低MDA,MPO,内毒素,TNFα和IL-6水平,并增加SOD水平,这在统计学上具有统计学意义(P <0.05);水分检测的结果是,肺组织和肠组织中的水分含量明显高于对照组,但是,经PNS处理后,水分含量显着降低。 HE染色可见肺组织细胞的形态变化。 Western blot检测显示,肺组织和肠组织中,模型组ICAM-1水平明显高于对照组,PNS组低于模型组。 PNS可以增加SOD活性,降低MDA,内毒素和MPO的水平,降低TNFα和IL-6的表达,并降低肺组织和肠组织的水分含量。因此,PNS通过抗氧化应激和抗炎途径对大鼠失血性休克模型具有保护作用,而ICAM-1可能在该机制中起重要作用。

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